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| Status |
Public on Aug 31, 2021 |
| Title |
Single cell analysis reveals intra-tumor heterogeneity and microenvironment for clear cell renal cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we performed 3’ transcriptome single cell sequencing on 7 ccRCC patients’ tumor and matched 5 adjacent normal samples. We identified two different functional subgroups in ccRCC tumor epithelial cells and two different gene transcriptional programs that were associated with survival across a TCGA cohort. We found important transcriptional factor regulons that played roles in ccRCC progression. We identified macrophage and T cell clusters whose higher infiltration in tumor was associated with overall survival. We also discovered some potentially targetable inhibitory interactions among tumor epithelial cells, the infiltrating T cell and myeloid compartments. Collectively, our data provided cell clusters and gene sets for predict clinical outcome and potential targets for ccRCC therapy.
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| Overall design |
Single cell mRNA profiles of 7 primary tumors and correspongding para-tumors from 7 patients with celar cell cancer were generated by 10X Genomics plartform, without technical replication, and sequenced by NovaSeq 6000 (Illumina)
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| |
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| Contributor(s) |
Zhang M, Wang J, Gao W |
| Citation(s) |
35605062 |
| |
| Submission date |
Aug 21, 2020 |
| Last update date |
Aug 29, 2023 |
| Contact name |
Jia Wang |
| E-mail(s) |
wj860520@163.com
|
| Phone |
15821724825
|
| Organization name |
SJTU CHINA
|
| Street address |
Huashan Rd. 1940, build 3. Shanghai,
|
| City |
Shanghai |
| State/province |
Shanghai China |
| ZIP/Postal code |
200017 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA658558 |
| SRA |
SRP278389 |
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