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| Status |
Public on Aug 22, 2020 |
| Title |
circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Circular RNAs (circRNAs) are a class of covalently closed single-stranded RNAs that have been demonstrated to involve in cancer progression. Here, we identify circNDUFB2 is downregulated in non-small cell lung cancer (NSCLC) tissues, and it negatively correlates with the malignant features. Elevated circNDUFB2 inhibits growth and metastasis of NSCLC cells. circNDUFB2 functions as a scaffold to enhance interaction of TRIM25 with IGF2BPs by forming a TRIM25/circNDUFB2/IGF2BPs ternary complex. The resultant ternary complex facilitates ubiquitination degradation of IGF2BPs, and N6-methyladenosine (m6A) modification of circNDUFB2 increases ubiquitination efficiency of TRIM25 for IGF2BPs. Moreover, circNDUFB2 can be recognized by RIG-I, then activates RIG-I-MAVS signaling cascades and recruits immune cells into tumor microenvironment (TME). Our data provide evidences that circNDUFB2 participates in degradation of IGF2BPs and activation of anti-tumor immunity in NSCLC progression. These findings reveal a dual-role pattern of circNDUFB2 action involved in protein ubiquitination degradation and cellular immune response.
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| |
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| Overall design |
mRNA profiles in A549 cells with circNDUFB2 overexpression (circNDUFB2) versus control (Vector)
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| |
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| Contributor(s) |
Li B, Zhu L, Jiang L, Qin W |
| Citation(s) |
33436560 |
| |
| Submission date |
Aug 21, 2020 |
| Last update date |
Jan 26, 2021 |
| Contact name |
Botai Li |
| E-mail(s) |
libotai@zju.edu.cn
|
| Phone |
15221287275
|
| Organization name |
Botai Li
|
| Street address |
Line 2200Xie-tu Road
|
| City |
shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA658508 |
| SRA |
SRP278367 |
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