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| Status |
Public on Mar 27, 2024 |
| Title |
in situ Hi-C profiles of DP thymocyltes from control and Suv39h1/Suv39h2 double knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
H3K9me3-dependent heterochromatin is critical for the silencing of repeat-rich pericentromeric regions and also has key roles in repressing lineage-inappropriate protein-coding genes for healthy cellular function. Here we investigate the disruption of genome organization in the absence of H3K9me3-dependent heterochromatin by performing in situ Hi-C in primary immune cells deficient in both Suv39h1 and Suv39h2 (Suv39DKO), the major mammalian histone methyltransferase enzymes which catalyse heterochromatic H3K9me3 deposition.
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| Overall design |
CD4+CD8+ double positive mouse thymocyte cells were extracted from two Suv39h1+/y Suv39h2+/- (control) mice and two Suv39h1-/y Suv39h2-/- double knock-out (Suv39DKO) mice and profiled by in situ Hi-C. All samples correspond to individual mice.
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| Contributor(s) |
Keenan CR, Coughlan HD, Smyth GK, Allan RS |
| Citation(s) |
38719473 |
| |
| Submission date |
Aug 14, 2020 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
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| Fax |
(+61 3) 9347 0852
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| URL |
http://www.wehi.edu.au
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| Organization name |
Walter and Eliza Hall Institute of Medical Research
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| Department |
Bioinformatics
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| Lab |
Smyth
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| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| GSM4728477 |
Suv39h1, Suv39h2 double knock-out, replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE156297 |
RNA-seq, LaminB1 ChIP-seq and in situ Hi-C of double positive thymocytes from control and Suv39h1/Suv39h2 double knockout mice |
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| Relations |
| BioProject |
PRJNA657285 |
| SRA |
SRP277564 |
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