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Series GSE156064 Query DataSets for GSE156064
Status Public on Aug 17, 2020
Title Total RNA sequencing of cultured splenic PKM2WT and PKM2KO NK cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PKM2 has recently been characterised in macrophages and T cells to play a crucial signalling role, impacting the transcription of downstream metabolic enzymes and effector molecules. We sought to test whether it plays a role in NK cells through whole RNA sequesncing of cells with or without PKM2.
Overall design PKM2 WT and PKM2 KO NK cells were expanded for 6 days with IL-15 and then treated with IL-2/12 or IL-2/12 + vehicle or TEPP-46 (50uM) (PBS) for 18 hours. Total RNA was extracted from 3 biological replicates for each group and was subject to total RNA sequencing.
Contributor(s) McVicar D
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Submission date Aug 11, 2020
Last update date Aug 17, 2020
Contact name Jessica Walls
Organization name National Cancer Institute
Street address Bulding 560, Rm 31-45
City Frederick
State/province MD
ZIP/Postal code 21701
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (12)
GSM4721812 WT IL-2/12 18hr biological replicate 1
GSM4721813 WT IL-2/12 18hr biological replicate 2
GSM4721814 WT IL-2/12 18hr biological replicate 3
BioProject PRJNA656569
SRA SRP277094

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Supplementary file Size Download File type/resource
GSE156064_DEG_2-12_Tepp_vs_2-12Veh_FC1.5_FDR0.05.xlsx 12.2 Kb (ftp)(http) XLSX
GSE156064_DEG_KO_2-12_18hr_vs_wt_2-12_18hr_FC_1.5_FDR_0.05.txt.gz 1.3 Kb (ftp)(http) TXT
GSE156064_RNASeq_NKcells_RPKM_for_GEO.xlsx 1.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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