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| Status |
Public on Aug 17, 2020 |
| Title |
Total RNA sequencing of cultured splenic PKM2WT and PKM2KO NK cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
PKM2 has recently been characterised in macrophages and T cells to play a crucial signalling role, impacting the transcription of downstream metabolic enzymes and effector molecules. We sought to test whether it plays a role in NK cells through whole RNA sequesncing of cells with or without PKM2.
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| Overall design |
PKM2 WT and PKM2 KO NK cells were expanded for 6 days with IL-15 and then treated with IL-2/12 or IL-2/12 + vehicle or TEPP-46 (50uM) (PBS) for 18 hours. Total RNA was extracted from 3 biological replicates for each group and was subject to total RNA sequencing.
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| Contributor(s) |
McVicar D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 11, 2020 |
| Last update date |
Aug 17, 2020 |
| Contact name |
Jessica Walls |
| E-mail(s) |
wallsj@tcd.ie
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| Organization name |
National Cancer Institute
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| Street address |
Bulding 560, Rm 31-45
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21701 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA656569 |
| SRA |
SRP277094 |
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