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Series GSE156022 Query DataSets for GSE156022
Status Public on Aug 10, 2023
Title RNA-seq of mouse embryonic kidney stromal cells with stabilized or deficient β-catenin expression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to identify β-catenin target genes in the developing renal stroma. We conducted a gene expression analysis in E12.5 and E13.5 renal stromal cells with either stabilized (β-catSO) or deficient (β-catSD) β-catenin expression. Expression of some candidate β-catenin targets was validated at the protein level by immunohistochemistry.
Overall design Stromal cells were isolated from E12.5 and E13.5 wild-type, β-catSO and β-catSD FoxD1Cre-eGFP mouse kidneys using fluorescence activated cell sorting (FACS). Three kidneys from each genotype were used for cell isolation. RNA profiles of the isolated cells were generated using the Illumina HiSeq system.
Contributor(s) Li A, Boivin F, Cunanan J, Dvorkin-Gheva A, Deacon E, Bridgewater D
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Submission date Aug 11, 2020
Last update date Aug 10, 2023
Contact name Darreb Bridgewater
Organization name McMaster University
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S 4K1
Country Canada
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (24)
GSM4720560 K-O-12-5-1
GSM4720561 K-O-12-5-2
GSM4720562 K-O-12-5-3
BioProject PRJNA656446
SRA SRP277026

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156022_Bridgewater_RNAseq_VOOM-TMM.csv.gz 1.7 Mb (ftp)(http) CSV
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Processed data are available on Series record

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