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Series GSE155638 Query DataSets for GSE155638
Status Public on Aug 25, 2021
Title Stage-resolved genome architecture maps throughout meiotic prophase link regional variations in chromosome organization with homolog alignment
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary During meiosis, chromosomes undergo extensive changes in structure and intranuclear positioning. How these chromosome organization changes occur and how they influence meiosis-specific chromosome events are not fully understood. Using Hi-C, we characterized chromosome architecture throughout mouse spermatogenesis at high temporal resolution. Our study revealed an intimate link between chromosome organization features and homolog pairing and alignment. We found that the meiotic chromosomes progressively reshape from TAD-like domains into linearly arranged loop arrays during prophase I. The transcriptionally active and inactive genomic regions exhibit distinct dynamics of loop growth, resulting in alternating domains consisting of shorter and longer chromosome loops. Such a domanial organization along meiotic chromosome axes is tightly correlated with the strength and precision of inter-homolog alignment. We further showed that a significant fraction of chromosomes near chromosome ends exhibit elevated inter-chromosomal association upon entering zygotene stage, while also exhibiting a higher degree of inter-homolog alignment. Using a mouse model defective in LINC complex component SUN1, we demonstrated that the prominent alignment of chromosome ends is dependent on the association of telomeres with the mechano-transducing LINC complex, but not the tethering of telomeres to the nuclear periphery. Taken together, our results suggest the 3D chromosome organization may provide a structural framework for the regulation of meiotic chromosome processes in higher eukaryotes.
 
Overall design In this study, we employed mouse spermatogenesis as a model system to investigate the spatial organization of mammalian meiotic chromosomes. By combining fluorescence-activated cell sorting (FACS) and spermatogenesis synchronization, we generated a complete roadmap of meiotic chromosome architecture throughout the meiotic prophase I.
 
Contributor(s) Bian Q, Zuo W
Citation(s) 34625553
Submission date Aug 03, 2020
Last update date Oct 27, 2021
Contact name Qian Bian
E-mail(s) qianbian@shsmu.edu.cn
Organization name Shanghai Jiao Tong University School of Medicine
Department Ninth People’s Hospital
Street address Pudong New District, Jinzun Rd, No.115
City Shanghai
State/province Shanghai
ZIP/Postal code 200125
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM4709265 L_1
GSM4709266 L_2
GSM4709267 Z_1
Relations
BioProject PRJNA650530
SRA SRP275681

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155638_D_10kb.cool.gz 501.3 Mb (ftp)(http) COOL
GSE155638_L_10kb.cool.gz 226.8 Mb (ftp)(http) COOL
GSE155638_P_10kb.cool.gz 386.0 Mb (ftp)(http) COOL
GSE155638_RAW.tar 1.5 Gb (http)(custom) TAR (of COOL)
GSE155638_Z_10kb.cool.gz 273.2 Mb (ftp)(http) COOL
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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