GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE155498 Query DataSets for GSE155498
Status Public on Mar 03, 2022
Title Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Biliary diseases can cause inflammation, fibrosis, bile duct destruction and eventually liver failure. There are no curative treatments for biliary disease except for liver transplantation. New therapies are urgently required.
We have purified human Biliary Epithelial Cells (hBEC) from discarded human livers. hBECs were tested as a cell therapy in a mouse model of biliary disease where the conditional deletion of Mdm2 in cholangiocytes causes senescence, biliary strictures and fibrosis.
hBEC are expandable, phenotypically stable and help restore biliary structure and function, highlighting their regenerative capacity and a potential alternative to liver transplantation for biliary disease.
Overall design Differential expression of CD133+ and CD133- human biliary epithelial cells from healthy (n=2) and steatotic (n=3) livers; Gene expression at different cell passages of CD133+ human biliary epithelial cells grown in vitro in two dimension-GMP compatible conditions (2D; n=4) and three dimensions (3D, organoids; n=5)
Contributor(s) Hallett JM, Ferreira-Gonzalez S, Man TY, Kilpatrick AM, Esser H, Thirlwell K, MacMillan M, Rodrigo-Torres D, Dwyer BJ, Gadd VL, Ashmore-Harris C, Lu W, Thomson J, Jansen MA, O'Duibhir E, Starkey Lewis PJ, Campana L, Aird R, Bate T, Fraser AR, Campbell JD, Oniscu GC, Hay D, Callanan A, Forbes SJ
Citation(s) 35245467
Submission date Jul 31, 2020
Last update date Mar 09, 2022
Contact name Stuart Forbes
Organization name The University of Edinburgh
Department Centre for Regenerative Medicine
Street address 5 Little France Drive
City Edinburgh
ZIP/Postal code EH16 4UU
Country United Kingdom
Platforms (2)
GPL21697 NextSeq 550 (Homo sapiens)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (19)
GSM4705315 Biliary epithelial cells - HL4 CD133+
GSM4705316 Biliary epithelial cells - HL4 CD133-
GSM4705317 Biliary epithelial cells - HL5 CD133+
BioProject PRJNA649950
SRA SRP274582

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155498_2d3d_gene_counts_matrix.txt.gz 469.6 Kb (ftp)(http) TXT
GSE155498_hBEC_gene_counts_matrix.txt.gz 428.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap