GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE155059 Query DataSets for GSE155059
Status Public on Jul 25, 2020
Title Characterization of lncRNA and mRNA Profiles in Rats With Diabetic Macroangiopathy
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Diabetic macroangiopathy is one of the most common serious complications of diabetes. Previous studies indicate that lncRNAs involved in the process of diabetes and another vascular disease. However, their detailed mechanism of the lncRNAs involved in diabetic macroangiopathy has not been well characterized. In the present study, we generated rat models of diabetic macroangiopathy induced by High fat. A total of 15 GK rats was constructed as a test group, along with 15 Wistar rats set as a control group, and thoracic aorta tissue from each group was collected. Whole genomic RNA sequencing was performed on thoracic aorta tissue;3223 novel lncRNAs and 20367 annotated lncRNAs were identified in thoracic aorta samples, and 864 lncRNAs were expressed differently in the test and control groups. Gene ontology term enrichment showed the apparent enrichment of inflammatory response and cell apoptosis, which consistent with the results of H&E Staining, TUNEL Assay, and ELISA; Extensive literature reveals inflammatory response and cell apoptosis play an important role in the process of diabetic macroangiopathy.The results of the present study indicated that lncRNAs, especiallyNrep.bSep08,Col5a1,aSep0,soygee.aSep08-unspliced,NONRATT013247.2,votar.aSep08-unspliced,etc,both participate in and mediate the process of inflammatory response,cell apoptosis.What’s more, Our research provides further insights into the understanding of the basic molecular mechanisms underlying diabetic macroangiopathy.
Overall design Our research showed that characterization of lncRNA and mRNA Profiles in Rats With Diabetic Macroangiopathy by High-throughput sequencing technology which was used to obtain the differential mRNA/lncRNA expression profiles of the thoracic aorta of each group of rats, further screen the differential mRNA/differential lncRNA target gene collection, and use GO enrichment and KEGG enrichment for biological analysis. Interpret the characterization of lncRNA and mRNA Profiles in Rats With Diabetic Macroangiopathy from the molecular level.
Contributor(s) yang c
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 24, 2020
Last update date Jul 25, 2020
Contact name chan yang
Organization name Hospital of Chengdu University of Traditional Chinese Medicine
Street address No 39 Shi-er-qiao Road
City Chengdu
ZIP/Postal code 610072
Country China
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (8)
GSM4694529 Thoracic aorta of control rat [C07]
GSM4694530 Thoracic aorta of control rat [C08]
GSM4694531 Thoracic aorta of control rat [C09]
BioProject PRJNA648366
SRA SRP273427

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155059_all.counts.g2_vs_g1.edgeR_GO_enrichment.txt.gz 274.3 Kb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_GO_level2.txt.gz 28.8 Kb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_KEGG_class.txt.gz 8.0 Kb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_KEGG_enrichment.txt.gz 20.0 Kb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_all.txt.gz 10.3 Mb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_differential.txt.gz 670.0 Kb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_differential_DOWN.txt.gz 351.7 Kb (ftp)(http) TXT
GSE155059_all.counts.g2_vs_g1.edgeR_differential_UP.txt.gz 361.8 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_diff_gene_GO_enrichment.txt.gz 631 b (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_diff_gene_GO_level2.txt.gz 789 b (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_diff_gene_KEGG_class.txt.gz 394 b (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_gene_GO_enrichment.txt.gz 8.8 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_gene_GO_level2.txt.gz 2.0 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_gene_KEGG_class.txt.gz 700 b (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_circRNA_parental_gene_KEGG_enrichment.txt.gz 821 b (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_target_diff_mRNA.txt.gz 116.4 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_target_diff_mRNA_GO_enrichment.txt.gz 34.4 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_target_diff_mRNA_GO_level2.txt.gz 4.7 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_target_diff_mRNA_KEGG_class.txt.gz 2.8 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_target_diff_mRNA_KEGG_enrichment.txt.gz 5.9 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_to_target_gene.txt.gz 3.0 Mb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_to_target_gene_GO_enrichment.txt.gz 266.2 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_to_target_gene_GO_level2.txt.gz 28.6 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_to_target_gene_KEGG_class.txt.gz 8.4 Kb (ftp)(http) TXT
GSE155059_g2_vs_g1_diff_lncRNA_to_target_gene_KEGG_enrichment.txt.gz 15.3 Kb (ftp)(http) TXT
GSE155059_lncRNA_count.g2_vs_g1.edgeR_all.txt.gz 2.7 Mb (ftp)(http) TXT
GSE155059_lncRNA_count.g2_vs_g1.edgeR_differential.txt.gz 88.1 Kb (ftp)(http) TXT
GSE155059_lncRNA_count.g2_vs_g1.edgeR_differential_DOWN.txt.gz 34.3 Kb (ftp)(http) TXT
GSE155059_lncRNA_count.g2_vs_g1.edgeR_differential_UP.txt.gz 55.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap