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Series GSE15417 Query DataSets for GSE15417
Status Public on May 03, 2009
Title Depletion of Erk1 and Erk2 MAP kinases in primary human keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We assessed the effect of RNAi-mediated MAP kinase cascade signaling blockade in primary human keratinocytes. Two sets of siRNA targeting different regions of the Erk1/2 genes were used, enabling identification of off-target siRNA effects.
Overall design Primary human keratinocytes were electroporated with scrambled control siRNA, a pair of siRNA oligomers against Erk1 and Erk2 (set A), or an independent pair of siRNA oligomers against Erk1 and Erk2 (set B). Four days after electroporation, RNA was harvested. Three biological replicates were performed for each of the three siRNA groups.
Contributor(s) Dumesic PA, Scholl FA, Barragan DI, Khavari PA
Citation(s) 19414607
Submission date Mar 26, 2009
Last update date Dec 06, 2018
Contact name Phillip Anthony Dumesic
Organization name Dana-Farber Cancer Institute
Street address 360 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (9)
GSM386967 Keratinocytes_scrambledsiRNA_rep1
GSM386969 Keratinocytes_scrambledsiRNA_rep2
GSM386970 Keratinocytes_scrambledsiRNA_rep3
BioProject PRJNA115823

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Supplementary file Size Download File type/resource
GSE15417_RAW.tar 19.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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