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| Status |
Public on Dec 22, 2021 |
| Title |
Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting [RRBS] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
We asked whether oocyte number could be amplified through parthenogenesis of mouse oocytes, without requiring creation of a paternal genome and a genetically unique genome. Parthenotes develop to a blastocyst-like stage, and from this parthenogenetic ESCs (pESCs) can be derived with high efficiency. Like ESCs, pESCs maintain unlimited self-renewal and pluripotency, as well as germline competence. Further, we demonstrate that their expression pattern of imprinted maternal genes resembles that observed in oocytes. pESCs can be directed to differentiate into primordial germ cell-like cells (PGCLCs) and form oocytes that produce fertile pups and reconstitute ovarian endocrine functions. The transcriptome and imprinting pattern of PGCLCs differentiated from pESCs more closely approximate those of in vivo produced embryonic PGCs, than PGCLCs produced from ESCs. Parthenogenesis offers a promising route for deriving PGCLCs and amplifying oocytes by faithfully maintaining maternal genes, without fertilization.
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| Overall design |
We performed RRBS assay to analysis the DNA methylation between female ESC and pESC, and also analysis the DNA methylation among E9.5PGC, E12.5PGC, female ESC-PGCLC and pESC-PGCLCs
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| |
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| Contributor(s) |
Tian C |
| Citation(s) |
34845589 |
| |
| Submission date |
Jul 07, 2020 |
| Last update date |
Dec 23, 2021 |
| Contact name |
Chenglei Tian |
| E-mail(s) |
chenglei.tian@helmholtz-munich.de
|
| Organization name |
Helmholtz Munich
|
| Lab |
Instirution of translational stem cell research
|
| Street address |
Ingolstädter Landstraße 1
|
| City |
Munich |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE154145 |
Amplification of Germ Cells through Parthenogenesis Faithfully Maintain Genomic Imprinting |
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| Relations |
| BioProject |
PRJNA644638 |
| SRA |
SRP270827 |
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