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Series GSE153818 Query DataSets for GSE153818
Status Public on Sep 29, 2020
Title scRNA-seq on C57BL/6J mice retina
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fresh retinatissues were harvested from 30 day old C57BL/6J mice according to published protocols. 3 retinas were pooled together to form one biological sample. Under a dissecting microscope, spring scissors were used to puncture the eye and remove the cornea, iris, and lens. The remaining eyecup had 4 radial incisions made every 90 degrees, resulting in a flat and open eye cup. The retina was then gently removed using curved tweezers and placed in a 1.5 ml microcentrifuge tube.
 
Overall design Fresh retinatissues were harvested from 30 day old C57BL/6J mice according to published protocols. 3 retinas were pooled together to form one biological sample. Under a dissecting microscope, spring scissors were used to puncture the eye and remove the cornea, iris, and lens. The remaining eyecup had 4 radial incisions made every 90 degrees, resulting in a flat and open eye cup. The retina was then gently removed using curved tweezers and placed in a 1.5 ml microcentrifuge tube.
 
Contributor(s) Kallestad L, Palczewski K
Citation(s) 32691052
Submission date Jul 05, 2020
Last update date Sep 29, 2020
Contact name Les Kallestad
Organization name University of California Irvine
Department Ophthalmology
Lab Krzysztof Palczewski
Street address 829 Health Sciences Rd
City Irvine
State/province CA
ZIP/Postal code 92617
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (1)
GSM4654742 WT_Retina
Relations
BioProject PRJNA644203
SRA SRP270068

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE153818_RAW.tar 19.6 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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