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Status |
Public on Dec 10, 2020 |
Title |
Gene expression differences among human natural killer cells and recombinant expression hosts |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
This study was designed to identify gene expression differences in activatable cells used to test therapeutic monoclonal antibody efficacy in vitro. We characterized the types of glycans attached to CD16a that were expressed by four cell types and compared these compositional differences to gene expression.
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Overall design |
At least three biological replicates of each cell type were performed. RNA was recovered from the primary human NK cells of three donors, and RNA was recovered from NK92, YTS and HEK293F cells grown in culture and analyzed by RNA-Seq.
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Contributor(s) |
Barb AW |
Citation(s) |
33310702 |
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Submission date |
Jul 02, 2020 |
Last update date |
Mar 11, 2021 |
Contact name |
Adam W. Barb |
E-mail(s) |
abarb@uga.edu
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Organization name |
University of Georgia
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Department |
Biochemistry and Molecular Biology
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Lab |
B302A
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Street address |
180 E. Green Street
|
City |
Athens |
State/province |
GA |
ZIP/Postal code |
30602 |
Country |
USA |
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Platforms (2) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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Samples (24)
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Relations |
BioProject |
PRJNA643836 |
SRA |
SRP269805 |