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Series GSE153638 Query DataSets for GSE153638
Status Public on Jul 02, 2020
Title RNA-seq analysis of rat neurons treated with shRNA-mediated Trank1 knockdown
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Genetic analyses for bipolar disorder (BPD) have achieved prominent success in Europeans in recent years, whereas its genetic basis in other populations remains relatively less understood. We herein report that the lead risk locus for BPD in European genome-wide association studies (GWAS), the single nucleotide polymorphism (SNP) rs9834970 near TRANK1 at 3p22 region, is also genome-wide significantly associated with BPD in 5,748 cases and 65,361 controls of East Asian origin. In this study, we performed RAN-seq analysis of cultured rat neurons treated with shRNA knockdown of Trank1.
Overall design Lentiviral constructs of two rat Trank1 shRNAs and a negative control shRNA were established to infect the wild-type rat neurons for 72h (n=3 per condition). Infection efficiency was estimated to be >80% and the mean expression of Trank1 was reduced by 43.4% and 35.7% respectively in cells infected with Trank1-shRNA#1 and Trank1-shRNA#2 relative to those infected with control shRNA.
Contributor(s) Li M, Cai X, Chang H, Li H
Citation(s) 32791513
Submission date Jul 01, 2020
Last update date Sep 30, 2020
Contact name Ming Li
Organization name Kunming Institute of Zoology, Chinese Academy of Sciences
Street address NO 32 Jiao-Chang Donglu
City Kunming
State/province Yunnan
ZIP/Postal code 650223
Country China
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (9)
GSM4648537 control shRNA-1
GSM4648538 control shRNA-2
GSM4648539 control shRNA-3
BioProject PRJNA643523
SRA SRP269554

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Supplementary file Size Download File type/resource
GSE153638_featureCountsFPKMMatrix.txt.gz 560.2 Kb (ftp)(http) TXT
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