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Series GSE152961 Query DataSets for GSE152961
Status Public on Jun 23, 2020
Title The RNA-binding proteins Roquin-1 and Regnase-1 interact and cooperate to prevent autoimmunity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This study aimed to analyze the transcriptomic changes occuring after knockout of alleles encoding for the RNA-binding proteins Roquin-1 and Roquin-2 (DKO), Regnase-1 (KO) or a combination of all three (TKO) in Th1 cells without or after restimulation with anti-CD3/28 antibodies.
Data analysis revealed that in unstimulated as well as restimulated T cells of all three knockout genotypes a number of genes is differentially expressed compared to WT cells, with the strongest deregulation seen in TKO cells. A comparison of gene expression changes of all three genotypes indicated that cooperativity of Roquin and Regnase-1 is the prevalent mode of target regulation.
Overall design RNA Seq of WT, DKO, KO or TKO of CD4 T cells without or with restimulation with anti-CD3/28 for 1h, 2h, 4h
Contributor(s) Heissmeyer V, Csaba G
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Submission date Jun 22, 2020
Last update date Jun 23, 2020
Contact name Vigo Heissmeyer
Organization name Ludwig-Maximilians-Universität
Department Institute for Immunology
Lab Heissmeyer lab
Street address Großhaderner Str. 9
City Planegg-Martinsried
State/province Bayern
ZIP/Postal code 82152
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (64)
GSM4630550 DKO_0h_rep1
GSM4630551 DKO_0h_rep2
GSM4630552 DKO_0h_rep3
BioProject PRJNA641085
SRA SRP268288

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Supplementary file Size Download File type/resource
GSE152961_roquin_regnase_tcell_knockout_timecourses_with_readinfos.csv.gz 16.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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