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Series GSE152856 Query DataSets for GSE152856
Status Public on Jan 11, 2024
Title YAP promotes cardiomyocyte cell cycle entry
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Specialized adult somatic cells, such as cardiomyocytes (CMs), are highly differentiated with poor renewal capacity, an integral reason underlying organ failure in disease and aging. Among the least renewable cells in the human body, CMs renew approximately 1% annually. Consistent with poor CM turnover, heart failure is the leading cause of death. Here, we show that an active version of the Hippo pathway effector YAP, termed YAP5SA, partially reprograms adult mouse CMs to a more fetal and proliferative state. One week after induction, 19% of CMs that enter S-phase do so twice, CM number increases by 40%, and YAP5SA lineage CMs couple to pre-existing CMs. Genomic studies showed that YAP5SA increases chromatin accessibility and expression of fetal genes, partially reprogramming long-lived somatic cells in vivo to a primitive, fetal-like, and proliferative state.
 
Overall design We conditionally expressed a Yap isoform incapable of being phosphorylated by Lats1/2, YAP5SA, in CMs using a mouse model that we described previously (Monroe et al., 2019). We then performed cardiac Drop-seq on Myh6-MerCreMer/+; YAP5SA-tg/+ (hereafter referred to as YAP5SA) mice, in addition to control animals.
 
Contributor(s) Hill MC
Citation(s) 38510108
Submission date Jun 19, 2020
Last update date Apr 02, 2024
Contact name Jong H Kim
E-mail(s) jonghwak@bcm.edu
Organization name Baylor College of Medicine
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM4628092 Control Drop-Seq CM-enriched
GSM4628093 Control Drop-Seq 2
GSM4628094 Control Drop-Seq 3
Relations
BioProject PRJNA640648
SRA SRP268028

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152856_YAP5SA_DropSeq.meta.data.csv.gz 990.9 Kb (ftp)(http) CSV
GSE152856_YAP5SA_DropSeq.raw.data.csv.gz 28.5 Mb (ftp)(http) CSV
GSE152856_YAP5SA_DropSeq.scale.data.csv.gz 40.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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