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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jan 11, 2024 |
Title |
YAP promotes cardiomyocyte cell cycle entry |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Specialized adult somatic cells, such as cardiomyocytes (CMs), are highly differentiated with poor renewal capacity, an integral reason underlying organ failure in disease and aging. Among the least renewable cells in the human body, CMs renew approximately 1% annually. Consistent with poor CM turnover, heart failure is the leading cause of death. Here, we show that an active version of the Hippo pathway effector YAP, termed YAP5SA, partially reprograms adult mouse CMs to a more fetal and proliferative state. One week after induction, 19% of CMs that enter S-phase do so twice, CM number increases by 40%, and YAP5SA lineage CMs couple to pre-existing CMs. Genomic studies showed that YAP5SA increases chromatin accessibility and expression of fetal genes, partially reprogramming long-lived somatic cells in vivo to a primitive, fetal-like, and proliferative state.
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Overall design |
We conditionally expressed a Yap isoform incapable of being phosphorylated by Lats1/2, YAP5SA, in CMs using a mouse model that we described previously (Monroe et al., 2019). We then performed cardiac Drop-seq on Myh6-MerCreMer/+; YAP5SA-tg/+ (hereafter referred to as YAP5SA) mice, in addition to control animals.
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Contributor(s) |
Hill MC |
Citation(s) |
38510108 |
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Submission date |
Jun 19, 2020 |
Last update date |
Apr 02, 2024 |
Contact name |
Jong H Kim |
E-mail(s) |
jonghwak@bcm.edu
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Organization name |
Baylor College of Medicine
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Street address |
One Baylor Plaza
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA640648 |
SRA |
SRP268028 |
Supplementary file |
Size |
Download |
File type/resource |
GSE152856_YAP5SA_DropSeq.meta.data.csv.gz |
990.9 Kb |
(ftp)(http) |
CSV |
GSE152856_YAP5SA_DropSeq.raw.data.csv.gz |
28.5 Mb |
(ftp)(http) |
CSV |
GSE152856_YAP5SA_DropSeq.scale.data.csv.gz |
40.8 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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