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| Status |
Public on Mar 02, 2021 |
| Title |
Antigen-driven EGR2 expression is required for exhausted CD8+ T cell stability and maintenance [RNA-seq II] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. Flow cytometric analysis indicated that EGR2 is expressed selectively within the progenitor exhausted subset, however a subpopulation of progenitor exhausted cells did not express in EGR2. To define the differences between the EGR2+ and EGR2- progenitor exhausted cells, GFP+ and GFP- polyclonal progenitor exhausted (ie. CD44int-hiPD-1+Slamf6+Tim3-) CD8+ T cells were sorted from Egr2-GFP reporter mice at day 20 p.i. with chronic LCMV-Cl13, and analysed by RNAseq. The resulting data demonstrated that GFP- progenitor cells have a more differentiated phenotype.
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| Overall design |
The transcriptome of Egr2-GFP reporter positive and negative progenitor exhausted CD8+ T cells was examined by RNAseq.
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| Contributor(s) |
Kelly M, Parish I |
| Citation(s) |
33986293 |
| |
| Submission date |
Jun 19, 2020 |
| Last update date |
May 20, 2021 |
| Contact name |
Ian Parish |
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE134710 |
Antigen-driven EGR2 expression is required for exhausted CD8+ T cell stability and maintenance |
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| Relations |
| BioProject |
PRJNA640640 |
| SRA |
SRP268023 |
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