NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE152757 Query DataSets for GSE152757
Status Public on Aug 13, 2020
Title Febrile temperature change modulates CD4 T cell differentiation via a TRPV channel-regulated Notch-dependent pathway 
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we report the transcriptomic differences between ex vivo CD4+ T cells (naïve CD4+ T Cells), CD4+ T cells activated with a mixture of anti-CD3 and anti-CD28 for 3 hours in vitro at 37ºC and 39ºC. At the higher temperature, we report the upregulation of protein folding machinery while downregulation of genes associated with mitochondrial oxidative phosphorylation and cytoplasmic proteiin synthesis.
 
Overall design CD4+ T cells were isolated from B6 mice spleen and subjected to activation at either 37ºC or 39ºC for three hours in presence of anti-CD3 and anti-CD28. Unactivated (naïve) cells as well as the activated cells were harvested and RNA was isolated using Trizol method. Total RNA was subjected to RNAseq analysis.
 
Contributor(s) Arimbasseri GA, Rath S, Umar D
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 18, 2020
Last update date Aug 15, 2020
Contact name G Aneeshkumar Arimbasseri
E-mail(s) aneesh@nii.ac.in
Phone +91 9971986746
Organization name National Institute of Immunology
Department Molecular Genetics Laboratory
Lab Molecular Genetics Laboratory
Street address Aruna Asaf Ali Marg
City New Delhi
State/province Delhi
ZIP/Postal code 110067
Country India
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM4625816 37_1
GSM4625817 37_2
GSM4625818 37_3
Relations
BioProject PRJNA640348
SRA SRP267841

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152757_RAW.tar 2.0 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap