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| Status |
Public on Nov 29, 2022 |
| Title |
Disease-specific exhaustion features and PD-1 immunotherapy responsiveness of antigen-specific CD8+ T cells at single-cell resolution [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We reported the CD8+ T cell exhaustion differences between chronic viral infection and tumor regarding their transcriptomic and epigenetic landscapes, immune checkpoint blockade responsiveness and underlying molecular mechanisms by using bulk RNA-seq, single-cell RNA-seq and single-cell ATAC-seq.
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| Overall design |
Exhausted P14 CD8+ T cells bearing a same TCR recognizing a same epitope presented in either chronic viral infection (LCMV-Cl13) or tumor (B16F10-GP) were harvested and performed with RNA- and ATAC-sequencing. Exhausted P14 CD8+ T cells were obtained from the spleens or lungs of chronic virus-infected mice and from the subcutaneous tumor tissues or metastatic lungs of tumor-engrafted mice. The chronic virus-infected or tumor-engrafted mice were treated with either anti-PD-L1 antibody or isotype antibody. As control, P14 CD8+ T cells from the spleens of acute virus-infected mice were also performed with RNA-sequencing.
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| Contributor(s) |
Ye L, Huang J, Cao G, Chen X |
| Citation missing |
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| |
| Submission date |
Jun 16, 2020 |
| Last update date |
May 24, 2023 |
| Contact name |
Jun Huang |
| E-mail(s) |
huangjun@uchicago.edu
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| Phone |
7737023218
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| Organization name |
University of Chicago
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| Department |
Institute for Molecular Engineering
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| Lab |
Huang Lab
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| Street address |
Eckhardt, 5640 S Ellis Ave
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE152628 |
Disease-specific exhaustion features and PD-1 immunotherapy responsiveness of antigen-specific CD8+ T cells at single-cell resolution |
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| Relations |
| BioProject |
PRJNA639836 |
| SRA |
SRP267562 |
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