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Series GSE152316 Query DataSets for GSE152316
Status Public on Jul 23, 2021
Title Etrolizumab treatment in Crohn's disease (Bergamot) cohort 1 RNA-seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Etrolizumab is an investigational monoclonal antibody that binds the β7 subunit of α4β7 and αEβ7 integrins. The relative contribution of α4β7 and αEβ7 to gut lymphocyte trafficking remains to be characterized. Here we show that αEβ7 is highly expressed on human intestinal CD8+ cytotoxic intraepithelial lymphocytes (IELs) and a small subset of proinflammatory CD4+ T cells. Dual blockade of α4β7 and αEβ7 reduced CD8+ T cell accumulation in the gut to a greater extent than single blockade of either pathway using a photo-convertible mouse model. αEβ7 blockade decreased T cell-epithelial interactions, increased the migratory speed and promoted egress of activated T cells. In Crohn’s disease patients treated with etrolizumab, a reduction of inflammatory genes and cytotoxic IEL gene signatures was observed. Concurrent blockade of α4β7 and αEβ7 increases reduction of cytotoxic IELs and inflammatory T cells in the gut mucosa through a stepwise inhibition of cell migration and tissue retention. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech.
 
Overall design RNA isolated from intestinal biopsies from a phase 3 randomized double-blind placebo-controlled 14-week trial of etrolizumab in Crohn's disease
Raw data not provided due to patient privacy concerns
 
Contributor(s) Keir M, Hackney JA
Citation(s) 34467254
Submission date Jun 11, 2020
Last update date Sep 08, 2021
Contact name Jason A Hackney
E-mail(s) hackney.jason@gene.com
Organization name Genentech, Inc.
Department Department of Bioinformatics and Computational Biology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (382)
GSM4612389 SAM24358697 Patient217 Y M Etrolizumab Week14 Ileum Inactive 1.08285527871738 GNE
GSM4612390 SAM24358698 Patient229 N F Placebo Week14 Colon Active 1.0143166169557 GNE
GSM4612391 SAM24358699 Patient218 Y F Etrolizumab Week14 Ileum Active 1.34173897146323 GNE
This SubSeries is part of SuperSeries:
GSE152321 Profiling intestinal T cell subsets and their dynamics in Crohn's disease after treatment with the anti-integrin therapeutic etrolizumab
Relations
BioProject PRJNA638957

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152316_RAW.tar 167.5 Mb (http)(custom) TAR (of TSV)
Raw data not provided for this record
Processed data provided as supplementary file

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