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| Status |
Public on Jan 15, 2021 |
| Title |
Intracellular pathways for lignin catabolism in white-rot fungi |
| Organisms |
Trametes versicolor; Gelatoporia subvermispora |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in Nature. While lignin depolymerization by WRF has been exhaustively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Here we employ 13C-labeling and systems biology approaches to demonstrate that two WRF, Trametes versicolor and Gelatoporia subvermispora, funnel lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These results provide insights into global carbon cycling in soil ecosystems, and furthermore establishes a foundation for employing WRF in simultaneous lignin depolymerization and bioconversion to bioproducts – a key step towards enabling a sustainable bioeconomy.
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| Overall design |
Examination of the effects of aromatic compounds, lignin, and lignocellulose on two different white rot fungi.
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| Contributor(s) |
Salvachua D, del Cerro C, Markillie LM, Gaffrey MJ, Mitchell HD |
| Citation missing |
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| |
| Submission date |
Jun 02, 2020 |
| Last update date |
Jan 17, 2021 |
| Contact name |
Davinia Salvachua |
| Organization name |
National Renewable Energy Laboratory
|
| Street address |
15013 Denver West Parkway
|
| City |
Golden |
| State/province |
CO |
| ZIP/Postal code |
80401 |
| Country |
USA |
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| Platforms (2) |
| GPL28618 |
NextSeq 550 (Trametes versicolor) |
| GPL28619 |
NextSeq 550 (Gelatoporia subvermispora) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA636664 |
| SRA |
SRP265586 |
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