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Series GSE151124 Query DataSets for GSE151124
Status Public on Apr 27, 2021
Title System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we deciphered the functions of Dhx36 and related molecular mechanisms in muscle stem cells and muscle regeneration. We found Dhx36 was highly induced in activated muscle stem cells during regeneration induced by injury. Conditional inactivation of Dhx36 in mouse muscle stem cells caused significant impaired regeneration, which was due to the dramatically decreased cell proliferation. Dhx36 was a well-known RNA helicase for binding/unwinding DNA/RNA G-quadruplexes and it was dominantly expressed in cytoplasm of proliferating myoblast. CLIP-seq was conducted to identify the mRNAs interacting with Dhx36 in myoblast cells. To examine whether Dhx36 regulates translation process, we also conducted polysome profiling followed by RNA-seq in WT and Dhx36 KO myoblast cells.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design 2 Dhx36 CLIP-seq, 6 polysome profiling experiments performed.

Refer to individual Series
 
Contributor(s) Wang H, Sun H, Chen X, Yuan J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 24, 2020
Last update date May 01, 2021
Contact name Jie Yuan
E-mail(s) jieyuan@link.cuhk.edu.hk
Organization name The Chinese University of Hong Kong
Department Orthopaedics and Traumatology
Lab Wang Lab
Street address Prince of Wales Hospital
City HONG KONG
State/province HONG KONG
ZIP/Postal code NA
Country China
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (8)
GSM4567042 Dhx36_CLIP_Bio_Rep1
GSM4567043 Dhx36_CLIP_Bio_Rep2
GSM4567044 WT_Total_mRNA
This SuperSeries is composed of the following SubSeries:
GSE151122 System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation [CLIP-seq]
GSE151123 System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation [Polysome profiling]
Relations
BioProject PRJNA634789

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151124_RAW.tar 10.2 Mb (http)(custom) TAR (of BED, FPKM_TRACKING)
SRA Run SelectorHelp

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