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Series GSE151123 Query DataSets for GSE151123
Status Public on Apr 27, 2021
Title System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation [Polysome profiling]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we deciphered the functions of Dhx36 and related molecular mechanisms in muscle stem cells and muscle regeneration. We found Dhx36 was highly induced in activated muscle stem cells during regeneration induced by injury. Conditional inactivation of Dhx36 in mouse muscle stem cells caused significant impaired regeneration, which was due to the dramatically decreased cell proliferation. Dhx36 was a well-known RNA helicase for binding/unwinding DNA/RNA G-quadruplexes and it was dominantly expressed in cytoplasm of proliferating myoblast. To examine whether Dhx36 regulates translation process, we conducted polysome profiling followed by RNA-seq in WT and Dhx36 KO myoblast cells.
 
Overall design 6 polysome profiling experiments performed.
 
Contributor(s) Wang H, Sun H, Chen X, Yuan J
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Submission date May 24, 2020
Last update date May 01, 2021
Contact name Jie Yuan
E-mail(s) jieyuan@link.cuhk.edu.hk
Organization name The Chinese University of Hong Kong
Department Orthopaedics and Traumatology
Lab Wang Lab
Street address Prince of Wales Hospital
City HONG KONG
State/province HONG KONG
ZIP/Postal code NA
Country China
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (6)
GSM4567044 WT_Total_mRNA
GSM4567045 WT_Polysome_associated_Bio_Rep1
GSM4567046 WT_Polysome_associated_Bio_Rep2
This SubSeries is part of SuperSeries:
GSE151124 System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation
Relations
BioProject PRJNA634791
SRA SRP263310

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151123_RAW.tar 10.1 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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