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Series GSE151107 Query DataSets for GSE151107
Status Public on Feb 20, 2021
Title eCLIP of Drosophila Asterix/Gtsf1 transfected in Drosophila ovarian somatic sheath (OSS) cells
Organism Drosophila melanogaster
Experiment type Other
Summary Asterix/Gtsf1 has been previously been shown to be involved in the silencing of retrotransposons in animal germ cells through the piRNA pathway. Using eCLIP and a custom informatic pipeline, we found that Asterix/Gtsf1 binds directly to RNAs and preferentially interacts with tRNAs as a class. Given the role of tRNAs as primers for Long Terminal Repeat (LTR) transposons, this work implicates Asterix/Gtsf1 as exploiting tRNA dependence to identify transposon transcripts and promote piRNA silencing.
 
Overall design eCLIP of Drosophila Asterix/Gtsf1 transfected in Drosophila ovarian somatic sheath (OSS) cells. The protein was expressed with a C-terminal TEV-Strep tag from the vector pAWG under the control of the act5c promoter.
 
Contributor(s) Ipsaro JJ, Joshua-Tor L
Citation(s) 33789107
NIH grant(s)
Grant ID Grant title Affiliation Name
F32 GM097888 Structural Basis for RNA-induced Silencing via the Piwi Clade of Argonautes COLD SPRING HARBOR LABORATORY JONATHAN JAMES IPSARO
Submission date May 24, 2020
Last update date Apr 01, 2021
Contact name Jonathan James Ipsaro
E-mail(s) jipsaro@cshl.edu
Organization name Cold Spring Harbor Laboratory
Lab Leemor Joshua-Tor
Street address 1 Bungtown Road
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
 
Platforms (1)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (6)
GSM4566708 Asterix-Strep eCLIP - non-crosslinked_input
GSM4566709 Asterix-Strep eCLIP - crosslinked_input1
GSM4566710 Asterix-Strep eCLIP - crosslinked_input2
This SubSeries is part of SuperSeries:
GSE151112 Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons
Relations
BioProject PRJNA634750
SRA SRP263175

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151107_040819_123456_enrichment.txt.gz 457.3 Kb (ftp)(http) TXT
GSE151107_RAW.tar 3.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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