|
Status |
Public on Feb 20, 2021 |
Title |
eCLIP of Drosophila Asterix/Gtsf1 transfected in Drosophila ovarian somatic sheath (OSS) cells |
Organism |
Drosophila melanogaster |
Experiment type |
Other
|
Summary |
Asterix/Gtsf1 has been previously been shown to be involved in the silencing of retrotransposons in animal germ cells through the piRNA pathway. Using eCLIP and a custom informatic pipeline, we found that Asterix/Gtsf1 binds directly to RNAs and preferentially interacts with tRNAs as a class. Given the role of tRNAs as primers for Long Terminal Repeat (LTR) transposons, this work implicates Asterix/Gtsf1 as exploiting tRNA dependence to identify transposon transcripts and promote piRNA silencing.
|
|
|
Overall design |
eCLIP of Drosophila Asterix/Gtsf1 transfected in Drosophila ovarian somatic sheath (OSS) cells. The protein was expressed with a C-terminal TEV-Strep tag from the vector pAWG under the control of the act5c promoter.
|
|
|
Contributor(s) |
Ipsaro JJ, Joshua-Tor L |
Citation(s) |
33789107 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
F32 GM097888 |
Structural Basis for RNA-induced Silencing via the Piwi Clade of Argonautes |
COLD SPRING HARBOR LABORATORY |
JONATHAN JAMES IPSARO |
|
|
Submission date |
May 24, 2020 |
Last update date |
Apr 01, 2021 |
Contact name |
Jonathan James Ipsaro |
E-mail(s) |
jipsaro@cshl.edu
|
Organization name |
Cold Spring Harbor Laboratory
|
Lab |
Leemor Joshua-Tor
|
Street address |
1 Bungtown Road
|
City |
Cold Spring Harbor |
State/province |
NY |
ZIP/Postal code |
11724 |
Country |
USA |
|
|
Platforms (1) |
GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
Samples (6)
|
GSM4566708 |
Asterix-Strep eCLIP - non-crosslinked_input |
GSM4566709 |
Asterix-Strep eCLIP - crosslinked_input1 |
GSM4566710 |
Asterix-Strep eCLIP - crosslinked_input2 |
|
This SubSeries is part of SuperSeries: |
GSE151112 |
Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons |
|
Relations |
BioProject |
PRJNA634750 |
SRA |
SRP263175 |