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Series GSE151074 Query DataSets for GSE151074
Status Public on May 23, 2020
Title Genome stabilization by RAD51 stimulatory compound-1 enhances efficiency of somatic cell nuclear transfer-mediated reprogramming and full-term development of cloned mouse embryos
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we observed that the activity of Rad 51, a key modulator of homologous recombination repair (HRR) is lower in somatic cell nuclear transfer (SCNT)-derived eggs than in conventional IVF, and found a significantly lower level of DNA double-strand breaks (DSBs) in SCNT eggs during reprogramming. To address this difference, supplementation with RS-1, an activator of Rad51, during the activation of SCNT eggs can increase RAD51 expression and DSB foci and thereby overcome the developmental block. This protocol finally increased the efficiency of SCNT reprogramming. Through subsequent single-cell RNA-seq analysis, we observed the reactivation of a large number of genes that were not expressed in SCNT-2-cell embryos by the upregulation of HRR, which may be related to overcoming the developmental block. Additionally, there may be an independent pathway involving histone demethylase that can reduce reprograming resistance regions. This technology can contribute to the production of comparable cell sources for regenerative medicine.
 
Overall design Here we perform RNA-Seq experiment in RS-1-treated mouse 2-cell embryos.
 
Contributor(s) Lee DR, Lee AR, Hong K
Citation(s) 34021643
Submission date May 22, 2020
Last update date Jul 14, 2021
Contact name Dong Ryul Lee
E-mail(s) drleedr@cha.ac.kr
Organization name CHA University
Street address 335, Pangyo-ro
City Seongnam-si
State/province Gyeonggi-do
ZIP/Postal code 13488
Country South Korea
 
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (46)
GSM4565898 IVF 2-cell Rep1
GSM4565899 IVF 2-cell Rep2
GSM4565900 SCNT 2-cell Rep1
Relations
BioProject PRJNA634550
SRA SRP263010

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Supplementary file Size Download File type/resource
GSE151074_bulkseq_expression_table.txt.gz 451.7 Kb (ftp)(http) TXT
GSE151074_scseq_expression_table.txt.gz 831.1 Kb (ftp)(http) TXT
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