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| Status |
Public on Jun 22, 2020 |
| Title |
Control of gene expression mRNA N6-methyladenosine in pro-B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Other
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| Summary |
N6-methyladenosine (m6A) is the most abundant internal modification in eukaryotic messenger RNA (mRNA). In mice, loss of METTL14, the key m6A writer component, severely blocks B cell development. In this group of RNA sequencing data, we 1. conducted RNA-seq on WT and METTL14 KO pro-B cells (sample 1-10); 2. performed m6A-seq in WT pro-B cells (sample 11-14) and 3. conducted YTHDF2 RIP -seq in WT pro-B cells (sample 15-18). Our data supported that m6A repressed a group of genes in pro-B cells via its reader YTHDF2.
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| Overall design |
Pro-B cells were sorted as CD19+B220midCD2-CD43hismallIg(kappa)/Ig(lambda)- cells from mice and subject to sequencing as described in summary.
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| Contributor(s) |
Yu X, He C, Zheng Z |
| Citation(s) |
32610122 |
| |
| Submission date |
May 22, 2020 |
| Last update date |
Jul 06, 2020 |
| Contact name |
Chuan He |
| E-mail(s) |
chuanhe@uchicago.edu
|
| Organization name |
University of Chicago
|
| Department |
Chemistry
|
| Street address |
929 E 57th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA634546 |
| SRA |
SRP262999 |
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