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Status |
Public on Sep 01, 2021 |
Title |
Mechanistic dissection of dominant AIRE mutations in mouse models reveals evidence for AIRE auto-regulation [ATAC-seq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Using mouse models of several recessive and putative dominant-negative patient mutations we were able to dissect the mechanisms that underlie the recessive and dominant-negative capacities of the corresponding AIRE mutants, including a newly identified dominant-negative variant C446G We also identify an auto-regulatory mechanism by which AIRE negatively modulates its own expression.
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Overall design |
Chromatin accessibility profiles of mature medullary thymic epithelial cells (mTEChi) from young (4-7 weeks old) Aire +/+, Aire +/C313Y and Aire +/C442G mice generated in the study, as well as Aire-/-,were generated by deep sequencing, using the ATACseq method.
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Contributor(s) |
Jakub A, Yael G |
Citation(s) |
34477806 |
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Submission date |
May 21, 2020 |
Last update date |
Nov 29, 2021 |
Contact name |
Jakub Abramson |
E-mail(s) |
jakub.abramson@weizmann.ac.il
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Organization name |
Weizmann Institute of Science
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Department |
Department of Immunology
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Street address |
234 Herzl st.
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City |
Rehovot |
ZIP/Postal code |
7610001 |
Country |
Israel |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
GSE151013 |
Mechanistic dissection of dominant AIRE mutations in mouse models reveals evidence for AIRE auto-regulation |
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Relations |
BioProject |
PRJNA634368 |
SRA |
SRP262676 |