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Series GSE151011 Query DataSets for GSE151011
Status Public on Sep 01, 2021
Title Mechanistic dissection of dominant AIRE mutations in mouse models reveals evidence for AIRE auto-regulation [ATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Using mouse models of several recessive and putative dominant-negative patient mutations we were able to dissect the mechanisms that underlie the recessive and dominant-negative capacities of the corresponding AIRE mutants, including a newly identified dominant-negative variant C446G
We also identify an auto-regulatory mechanism by which AIRE negatively modulates its own expression.
 
Overall design Chromatin accessibility profiles of mature medullary thymic epithelial cells (mTEChi) from young (4-7 weeks old) Aire +/+, Aire +/C313Y and Aire +/C442G mice generated in the study, as well as Aire-/-,were generated by deep sequencing, using the ATACseq method.
 
Contributor(s) Jakub A, Yael G
Citation(s) 34477806
Submission date May 21, 2020
Last update date Nov 29, 2021
Contact name Jakub Abramson
E-mail(s) jakub.abramson@weizmann.ac.il
Organization name Weizmann Institute of Science
Department Department of Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 7610001
Country Israel
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM4564076 Aire_KO_B6_1
GSM4564077 Aire_KO_B6_2
GSM4564078 Aire_WT_B6_1
This SubSeries is part of SuperSeries:
GSE151013 Mechanistic dissection of dominant AIRE mutations in mouse models reveals evidence for AIRE auto-regulation
Relations
BioProject PRJNA634368
SRA SRP262676

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151011_RAW.tar 18.0 Mb (http)(custom) TAR (of BED, BROADPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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