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Series GSE150701 Query DataSets for GSE150701
Status Public on Oct 05, 2020
Title Specific ectodermal enhancers control the expression of Hoxc genes in developing mammalian teguments [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Vertebrate Hox genes are key players in the establishment of structures during the development of the main body axis. Subsequently, they play important roles either in organizing secondary axial structures such as the appendages, or during homeostasis in postnatal stages and adulthood. Here we set up to analyze their elusive function in the ectodermal compartment, using the mouse limb bud as a model. We report that the HoxC gene cluster was globally co-opted to be transcribed in the distal limb ectoderm, where it is activated following the rule of temporal colinearity. These ectodermal cells subsequently produce various keratinized organs such as nails or claws. Accordingly, deletion of the HoxC cluster led to mice lacking nails (anonychia) and also hairs (alopecia), a condition stronger than the previously reported loss of function of Hoxc13, which is causative of the ectodermal dysplasia 9 (ECTD9) syndrome in human patients. We further identified, in mammals only, two ectodermal-specific enhancers located upstream the gene cluster, which act synergistically to regulate Hoxc genes in these ectodermal organs. Deletion of these enhancers alone or in combination revealed a strong quantitative component in the regulation of these genes in the ectoderm, suggesting that these two enhancers may have evolved along with mammals to provide the level of HOXC proteins necessary for the full development of hairs and nails.
 
Overall design RNA-seq datasets of E9.5 to E12.5 distal part of forelimb embryos separated into mesoderm and ectoderm
 
Contributor(s) Fernandez-Guerrero M, Lopez-Delisle L, Duboule D, Ros M
Citation(s) 33199643
Submission date May 15, 2020
Last update date Jun 30, 2021
Contact name Lucille Lopez-Delisle
E-mail(s) lucille.delisle@epfl.ch
Organization name EPFL
Street address Station 19
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (16)
GSM4556930 MES_E9.5_rep1  
GSM4556931 MES_E9.5_rep2
GSM4556932 MES_E10.5_rep1  
This SubSeries is part of SuperSeries:
GSE150702 Specific ectodermal enhancers control the expression of Hoxc genes in developing mammalian teguments
Relations
BioProject PRJNA633175
SRA SRP261910

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150701_AllCufflinks_Simplified.txt.gz 2.2 Mb (ftp)(http) TXT
GSE150701_Counts.txt.gz 1.5 Mb (ftp)(http) TXT
GSE150701_RAW.tar 1.1 Gb (http)(custom) TAR (of BEDGRAPH, TXT)
GSE150701_normalized_ECT095_neq2_Uniq_fwd.bw 69.5 Mb (ftp)(http) BW
GSE150701_normalized_ECT095_neq2_Uniq_rev.bw 69.2 Mb (ftp)(http) BW
GSE150701_normalized_ECT105_neq2_Uniq_fwd.bw 60.2 Mb (ftp)(http) BW
GSE150701_normalized_ECT105_neq2_Uniq_rev.bw 60.3 Mb (ftp)(http) BW
GSE150701_normalized_ECT115_neq2_Uniq_fwd.bw 85.4 Mb (ftp)(http) BW
GSE150701_normalized_ECT115_neq2_Uniq_rev.bw 85.4 Mb (ftp)(http) BW
GSE150701_normalized_ECT125_neq2_Uniq_fwd.bw 83.8 Mb (ftp)(http) BW
GSE150701_normalized_ECT125_neq2_Uniq_rev.bw 83.8 Mb (ftp)(http) BW
GSE150701_normalized_MES095_neq2_Uniq_fwd.bw 74.3 Mb (ftp)(http) BW
GSE150701_normalized_MES095_neq2_Uniq_rev.bw 73.2 Mb (ftp)(http) BW
GSE150701_normalized_MES105_neq2_Uniq_fwd.bw 83.3 Mb (ftp)(http) BW
GSE150701_normalized_MES105_neq2_Uniq_rev.bw 82.5 Mb (ftp)(http) BW
GSE150701_normalized_MES115_neq2_Uniq_fwd.bw 77.6 Mb (ftp)(http) BW
GSE150701_normalized_MES115_neq2_Uniq_rev.bw 76.7 Mb (ftp)(http) BW
GSE150701_normalized_MES125_neq2_Uniq_fwd.bw 79.3 Mb (ftp)(http) BW
GSE150701_normalized_MES125_neq2_Uniq_rev.bw 78.4 Mb (ftp)(http) BW
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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