|
| Status |
Public on May 13, 2020 |
| Title |
DNA Double-Strand Breaks Induce H2Ax Phosphorylation Domains in a Contact-Dependent Manner |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Efficient repair of DNA double-strand breaks (DSBs) requires a coordinated DNA Damage Response (DDR), which includes phosphorylation of histone H2Ax, forming γH2Ax. This histone modification spreads beyond the DSB into neighboring chromatin, generating a DDR platform that protects against end disassociation and degradation, minimizing chromosomal rearrangements. However, mechanisms that determine the breadth and intensity of γH2Ax domains remain unclear. Here, we show that chromosomal contacts of a DSB site are the primary determinants for γH2Ax landscapes. DSBs that disrupt a topological border permit extension of γH2Ax domains into both adjacent compartments. In contrast, DSBs near a border produce highly asymmetric DDR platforms, with γH2Ax nearly absent from one broken end. Collectively, our findings lend insights into a basic DNA repair mechanism and how the precise location of a DSB may influence genome integrity.
|
| |
|
| Overall design |
Examination of 2 different chromatin modification in 3 cell types
Examination of chromatin topology by Hi-C in 3 different types and 3 different conditions
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| |
|
| Contributor(s) |
Collins PL, Hayer K, Oltz E |
| Citation(s) |
32572033 |
| |
| Submission date |
May 12, 2020 |
| Last update date |
Jun 29, 2020 |
| Contact name |
Patrick Leonard Collins |
| E-mail(s) |
patrick.collins@osumc.edu
|
| Phone |
6156683538
|
| Organization name |
Ohio State University
|
| Department |
Microbial Infection and Immunity
|
| Street address |
770 BRT, 460 W 12th Ave
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA631960 |
| SRA |
SRP261290 |
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