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Series GSE150384 Query DataSets for GSE150384
Status Public on May 13, 2020
Title DNA Double-Strand Breaks Induce H2Ax Phosphorylation Domains in a Contact-Dependent Manner
Organism Mus musculus
Experiment type Other
Summary Efficient repair of DNA double-strand breaks (DSBs) requires a coordinated DNA Damage Response (DDR), which includes phosphorylation of histone H2Ax, forming γH2Ax. This histone modification spreads beyond the DSB into neighboring chromatin, generating a DDR platform that protects against end disassociation and degradation, minimizing chromosomal rearrangements. However, mechanisms that determine the breadth and intensity of γH2Ax domains remain unclear. Here, we show that chromosomal contacts of a DSB site are the primary determinants for γH2Ax landscapes. DSBs that disrupt a topological border permit extension of γH2Ax domains into both adjacent compartments. In contrast, DSBs near a border produce highly asymmetric DDR platforms, with γH2Ax nearly absent from one broken end. Collectively, our findings lend insights into a basic DNA repair mechanism and how the precise location of a DSB may influence genome integrity.
Overall design Examination of 2 different chromatin modification in 3 cell types
Examination of chromatin topology by Hi-C in 3 different types and 3 different conditions
Contributor(s) Collins PL, Hayer K, Oltz E
Citation(s) 32572033
Submission date May 12, 2020
Last update date Jun 29, 2020
Contact name Patrick Leonard Collins
Phone 6156683538
Organization name Ohio State University
Department Microbial Infection and Immunity
Street address 770 BRT, 460 W 12th Ave
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (32)
GSM4548026 1_pH2Ax_6312_RNP-eB
GSM4548027 2_pH2Ax_6312_RNP-MycPro
GSM4548028 3_pH2Ax_p5424_RNP-eB
BioProject PRJNA631960
SRA SRP261290

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Supplementary file Size Download File type/resource
GSE150384_RAW.tar 62.4 Gb (http)(custom) TAR (of BW, HIC)
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Raw data are available in SRA
Processed data provided as supplementary file

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