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Series GSE150353 Query DataSets for GSE150353
Status Public on Nov 01, 2020
Title A histone H3.3K36M mutation in mice causes imbalance of histone modifications and defects in chondrocyte differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Histone lysine-to-methionine (K-to-M) mutations have been identified as driver mutations in human cancers. Interestingly, these ‘oncohistone’ mutations inhibit the activity of histone methyltransferases. Therefore, they can potentially be used as versatile tools to investigate the roles of histone modifications. In this study, we generated a genetically engineered mouse line in which an H3.3K36M mutation could be induced in the endogenous H3f3b gene. Since H3.3K36M has been identified as a causative mutation of human chondroblastoma, we induced this mutation in the chondrocyte lineage in mouse embryonic limbs. We found that H3.3K36M causes a global reduction in H3K36me2 and defects in chondrocyte differentiation. Importantly, the reduction of H3K36me2 was accompanied by a collapse of normal H3K27me3 distribution. Furthermore, the changes in H3K27me3, especially the loss of H3K27me3 at gene regulatory elements, were associated with the mis-regulated expression of a set of genes important for limb development, including HoxA cluster genes. Thus, through the in vivo induction of the H3.3K36M mutation, we reveal the importance of maintaining the balance between H3K36me2 and H3K27me3 during chondrocyte differentiation and limb development.
 
Overall design Refer to individual Series
 
Citation(s) 33135541
Submission date May 11, 2020
Last update date Feb 08, 2021
Contact name Takashi Ishiuchi
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Epigenetics and Development
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (28)
GSM4547349 Control input rep1
GSM4547350 Control input rep2
GSM4547351 H3.3K36M input rep1
This SuperSeries is composed of the following SubSeries:
GSE150351 A histone H3.3K36M mutation in mice causes imbalance of histone modifications and defects in chondrocyte differentiation [ChIP-seq]
GSE150352 A histone H3.3K36M mutation in mice causes imbalance of histone modifications and defects in chondrocyte differentiation [RNA-seq]
Relations
BioProject PRJNA631838

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150353_RAW.tar 995.7 Mb (http)(custom) TAR (of TDF)
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