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Series GSE150351 Query DataSets for GSE150351
Status Public on Nov 01, 2020
Title A histone H3.3K36M mutation in mice causes imbalance of histone modifications and defects in chondrocyte differentiation [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Histone lysine-to-methionine (K-to-M) mutations have been identified as driver mutations in human cancers. Interestingly, these ‘oncohistone’ mutations inhibit the activity of histone methyltransferases, and, therefore, they can potentially be used as versatile tools to investigate the roles of histone modifications. In this study, we created a conditional knock-in mouse line in which a H3.3K36M mutation can be induced in the endogenous H3.3 gene. Since the H3.3K36M mutation has been identified as a causative mutation of human chondroblastoma, we induced this mutation in the chondrocyte lineage in mouse embryonic limbs. We found that H3.3K36M causes global reduction of H3K36me2 and defects in chondrocyte differentiation. Importantly, the reduction of H3K36me2 was accompanied by a collapse of normal H3K27me3 distribution. Furthermore, the changes in H3K27me3, especially the loss of H3K27me3 at gene regulatory elements, were associated with misregulated expression of a set of genes important for limb development including HoxA cluster genes. Thus, taking advantage of the in vivo induction of H3.3K36M mutation, we reveal the importance of a counterbalance between H3K36me2 and H3K27me3 for chondrocyte differentiation and limb development.
Overall design ChIP-seq for H3K36me2, H3K36me3, and H3K27me3 were performed in mouse E14.5 tibial midshafts. mRNA-seq was performed in mouse E14.5 tibial midshafts and ends.
Contributor(s) Ishiuchi T, Abe S
Citation(s) 33135541
Submission date May 11, 2020
Last update date Feb 08, 2021
Contact name Takashi Ishiuchi
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Epigenetics and Development
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (16)
GSM4547349 Control input rep1
GSM4547350 Control input rep2
GSM4547351 H3.3K36M input rep1
This SubSeries is part of SuperSeries:
GSE150353 A histone H3.3K36M mutation in mice causes imbalance of histone modifications and defects in chondrocyte differentiation
BioProject PRJNA631842
SRA SRP261200

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Supplementary file Size Download File type/resource 276.1 Mb (ftp)(http) BW 260.0 Mb (ftp)(http) BW 271.4 Mb (ftp)(http) BW 185.0 Mb (ftp)(http) BW 269.4 Mb (ftp)(http) BW 225.0 Mb (ftp)(http) BW 257.4 Mb (ftp)(http) BW 174.1 Mb (ftp)(http) BW
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Processed data are available on Series record

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