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Series GSE150291 Query DataSets for GSE150291
Status Public on Oct 01, 2020
Title Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigation the role of PTH and Kindlin-2 in bone development, we performed single-cell RNA-sequencing. From Con-veh, Con-PTH, cKO-veh, cKO-PTH, we profiled more than 20k single cells, including multi-potent mesenchymal stromal cells (MSC), osteoprogenitors, osteoblasts, chondrocytes, fibroblasts, endothelial cells, smooth muscle cells, skeletal muscle cells, pericytes, and schwann cells. We found proportion of part of these cells were significant altered by PTH or Kindlin-2 loss, especially for MSC, osteoblast, chondrocyte, and fibroblast. Transcriptomic analysis revealed gene expression was dramatically regulated by PTH or Kindlin-2 loss.
Overall design 3-month-old Kindlin-2f/f (control, n=6) and Dmp1-Cre; Kindlin-2f/f (cKO, n=6) mice were treated with or without PTH daily continuously for 28d. Mice were sacrificed after the last PTH injection. Non-hematopoietic cells from bone marrow were extracted for single-cell RNA-sequencing
Contributor(s) Fu X, Zhou B, Xiao G
Citation(s) 33361757
Submission date May 11, 2020
Last update date Dec 29, 2020
Contact name Guozhi Xiao
Organization name Southern University of Science and Technology
Department Biology
Street address No. 1088, Xueyuan Road
City Shen Zhen
State/province Guang Dong
ZIP/Postal code 518055
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM4546352 control veh
GSM4546353 control PTH
GSM4546354 cKO veh
BioProject PRJNA631725
SRA SRP261116

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Supplementary file Size Download File type/resource
GSE150291_RAW.tar 453.9 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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