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Status |
Public on Oct 01, 2020 |
Title |
Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To investigation the role of PTH and Kindlin-2 in bone development, we performed single-cell RNA-sequencing. From Con-veh, Con-PTH, cKO-veh, cKO-PTH, we profiled more than 20k single cells, including multi-potent mesenchymal stromal cells (MSC), osteoprogenitors, osteoblasts, chondrocytes, fibroblasts, endothelial cells, smooth muscle cells, skeletal muscle cells, pericytes, and schwann cells. We found proportion of part of these cells were significant altered by PTH or Kindlin-2 loss, especially for MSC, osteoblast, chondrocyte, and fibroblast. Transcriptomic analysis revealed gene expression was dramatically regulated by PTH or Kindlin-2 loss.
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Overall design |
3-month-old Kindlin-2f/f (control, n=6) and Dmp1-Cre; Kindlin-2f/f (cKO, n=6) mice were treated with or without PTH daily continuously for 28d. Mice were sacrificed after the last PTH injection. Non-hematopoietic cells from bone marrow were extracted for single-cell RNA-sequencing
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Contributor(s) |
Fu X, Zhou B, Xiao G |
Citation(s) |
33361757 |
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Submission date |
May 11, 2020 |
Last update date |
Dec 29, 2020 |
Contact name |
Guozhi Xiao |
E-mail(s) |
xiaogz@sustech.edu.cn
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Organization name |
Southern University of Science and Technology
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Department |
Biology
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Street address |
No. 1088, Xueyuan Road
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City |
Shen Zhen |
State/province |
Guang Dong |
ZIP/Postal code |
518055 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (4)
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Relations |
BioProject |
PRJNA631725 |
SRA |
SRP261116 |
Supplementary file |
Size |
Download |
File type/resource |
GSE150291_RAW.tar |
453.9 Mb |
(http)(custom) |
TAR (of TAR) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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