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| Status |
Public on May 01, 2023 |
| Title |
Effect of EZH2 inhibition on ARID1A mutant bladder cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
ARID1A, a subunit of SWI/SNF chromatin remodeling complex. SWI/SNF complex can regulate expression of genes involved in vital biological processes such as cell cycle, DNA damage repair and development. ARID1A is known to have high mutation rate in human cancers including bladder cancer, leading to its loss of function. Publicly available whole exome sequencing data for muscle invasive and non-muscle invasive bladder cancers, show fraction of tumors with truncated ARID1A. Thus identifying therapeutic strategies for ARID1A mutant cancers is of high importance.
EZH2, a histone methyltransferase is known to over-express and play pivotal role in aggressive bladder cancer. Our preliminary studies show that treatment of EZH2 inhibitor (GSK126) on ARID1A mutant bladder cancer cells significantly reduced cancer cell viability, invasion and colony formation relative to wild type ARID1A containing cells. Here, we performed microarray experiments to assess the effect of EZH2 inhibitor on global transcriptome of both ARID1A mutant and wild type bladder cancer cell line.
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| Overall design |
Two color microarray experiments were performed considering DMSO treated and GSK126 (EZH2 inhibitor) treated bladder cancer cells. The study included two ARID1A mutant (HT1197, HT1376) and two ARID1A wildtype (T24 and 5637) bladder cancer cell lines.
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| Contributor(s) |
Chakravarthi BV, Chandrashekar DS, Varambally S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 11, 2020 |
| Last update date |
May 03, 2023 |
| Contact name |
Sooryanarayana Varambally |
| E-mail(s) |
svarambally@uabmc.edu
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| Organization name |
University of Alabama
|
| Department |
Pathology
|
| Street address |
1824 6th Avenue S
|
| City |
Birmingham |
| State/province |
Alabama |
| ZIP/Postal code |
35294-0007 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| GSM4544016 |
DMSO vs GSK126 treatment of HT1197 cells |
| GSM4544017 |
DMSO vs GSK126 treatment of HT1376 cells |
| GSM4544018 |
DMSO vs GSK126 treatment of T24 cells |
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| Relations |
| BioProject |
PRJNA631659 |
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