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| Status |
Public on Oct 13, 2021 |
| Title |
Single cell transcriptome of human alveolar epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine the ability of human AEC2s to transdifferentiate in vitro, we performed single cell mRNA sequencing using 10X Genomics platform of freshly sorted AEC2s and several timepoints in a 3D organoid co-culture (AEC2s with primary human mesenchyme) system.
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| |
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| Overall design |
Freshly sorted human AEC2s were co-cultured with primary human mesenchyme in a 3D organoids. At day 7, day 14, and day 21, epithelial cells and mesenchymal cells were harvested and sequenced via 10X Genomics.
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| |
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| Contributor(s) |
Kathiriya JJ, Wang C, Chapman HA, Peng T |
| Citation(s) |
34969962 |
| |
| Submission date |
May 07, 2020 |
| Last update date |
Jan 31, 2022 |
| Contact name |
Jaymin J Kathiriya |
| E-mail(s) |
jaymin.kathiriya@mssm.edu
|
| Organization name |
University of California San Francisco
|
| Department |
Medicine
|
| Lab |
Chapman Lab
|
| Street address |
513 Parnassus Ave
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94131 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA631019 |
| SRA |
SRP260482 |
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