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| Status |
Public on May 02, 2020 |
| Title |
RNA sequencing analysis of monocrotaline-induced pulmonary arterial hypertension |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Inflammation and immunity play a causal role in the pathogenesis of pulmonary vascular remodeling and pulmonary arterial hypertension (PAH). However, the pathways and mechanisms by which inflammation and immunity contribute to pulmonary vascular remodeling remain unknown. RNA sequencing was used to analyze the transcriptome in control and rats injected with monocrotaline (MCT) for 1, 2, 3 and 4 weeks. Using the transcriptional profiling of MCT-induced PAH coupled with bioinformatics analysis, we clustered the differentially expressed genes (DEGs) and chose the increased expression patterns associated with inflammatory and immune response. We found the enrichment of Toll-like receptor (TLR) and NOD-like receptor (NLR) pathways and identified NF-κB-mediated inflammatory and immune profiling in MCT-induced PAH. Pathway-based data integration and visualization showed the dysregulated TLR and NLR pathways, including increased expression of TLR2 and NLRP3, and their downstream molecules. Further analysis revealed that the activation of TLR and NLR pathways was associated with upregulation of damage-associated molecular patterns (DAMPs) and RIPK3-mediated necroptosis was involved in the generation of DAMPs in MCT-induced PAH. Collectively, we identify RIPK3-mediated necroptosis and its triggered TLR and NLR pathways in the progression of pulmonary vascular remodeling, thus, providing novel insights into the mechanisms underlying inflammation and immunity in the pathogenesis of PAH.
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| Overall design |
Lung transcriptional profiling of control and rats injected with monocrotaline (MCT) for 1, 2, 3 and 4 weeks was generated by the high-throughput RNA sequencing using Illumina 2000, n=5 control and n=3 for each MCT treatment week.
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| Contributor(s) |
Xiao G, Xie L |
| Citation(s) |
32860486, 34635097 |
| |
| Submission date |
May 01, 2020 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Genfa Xiao |
| E-mail(s) |
iesvev1986@aliyun.com
|
| Organization name |
First Affiliated Hospital of Fujian Medical University
|
| Lab |
Fujian hypertension research institute
|
| Street address |
chazhong road 20
|
| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350005 |
| Country |
China |
| |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (17)
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| Relations |
| BioProject |
PRJNA629790 |
| SRA |
SRP259927 |
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