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| Status |
Public on Sep 27, 2021 |
| Title |
Single Cell RNA Sequencing of Cultured Primary Tissue Cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using a single cell-RNA sequencing protocol from 10xGenomics, we compared so called cardiosphere-derived cells (CDCs, established protocol) to the main non-myocyte cell types of the adult heart: cardiac fibroblasts (CFs), smooth muscle cells (SMCs) and endothelial cells (ECs). Herby, CDCs were disclosed as a heterogenic cell type with clear similarities to cardiac non-myocyte cell types, particularly to CFs and SMCs. Beyond a high proliferation capacity and upregulation of immune-related genes, CDCs exhibited a strong extracellular matrix synthesizing ability and also some angiogenic potential. Subsequently, infant patient derived CDCs were compared to adult CDCs, revealing that infant-CDC upregulated developmental and angiogenic processes. This study represents the first characterization of CDCs by sc-RNAseq and unravels their age-dependent molecular differences.
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| Overall design |
1) Comparison of adult primary cardiac cell types to unravel the identity of CDCs. 2) Comparison of adult CDC to infant patient derived CDC.
10x Genomics protocol was used
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| |
|
| Contributor(s) |
Doppler SA, Kogan P, Tomar A |
| Citation(s) |
35258704 |
| Submission date |
May 01, 2020 |
| Last update date |
Mar 15, 2022 |
| Contact name |
Stefanie A Doppler |
| E-mail(s) |
doppler@dhm.mhn.de
|
| Organization name |
German Heart Center Munich at the Technische Universität München
|
| Department |
Department of Cardiovascular Surgery
|
| Street address |
Lazarettstraße 36
|
| City |
München |
| ZIP/Postal code |
80636 |
| Country |
Germany |
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| Platforms (2) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA629778 |
| SRA |
SRP259919 |
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