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Series GSE149699 Query DataSets for GSE149699
Status Public on Sep 27, 2021
Title Single Cell RNA Sequencing of Cultured Primary Tissue Cells.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Using a single cell-RNA sequencing protocol from 10xGenomics, we compared so called cardiosphere-derived cells (CDCs, established protocol) to the main non-myocyte cell types of the adult heart: cardiac fibroblasts (CFs), smooth muscle cells (SMCs) and endothelial cells (ECs). Herby, CDCs were disclosed as a heterogenic cell type with clear similarities to cardiac non-myocyte cell types, particularly to CFs and SMCs. Beyond a high proliferation capacity and upregulation of immune-related genes, CDCs exhibited a strong extracellular matrix synthesizing ability and also some angiogenic potential. Subsequently, infant patient derived CDCs were compared to adult CDCs, revealing that infant-CDC upregulated developmental and angiogenic processes. This study represents the first characterization of CDCs by sc-RNAseq and unravels their age-dependent molecular differences.
Overall design 1) Comparison of adult primary cardiac cell types to unravel the identity of CDCs. 2) Comparison of adult CDC to infant patient derived CDC.
10x Genomics protocol was used
Contributor(s) Doppler SA, Kogan P, Tomar A
Citation(s) 35258704
Submission date May 01, 2020
Last update date Mar 15, 2022
Contact name Stefanie A Doppler
Organization name German Heart Center Munich at the Technische Universität München
Department Department of Cardiovascular Surgery
Street address Lazarettstraße 36
City München
ZIP/Postal code 80636
Country Germany
Platforms (2)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (5)
GSM4509138 Adult Endothelial Cells [EC]
GSM4509139 Adult Cardiac Fibroblast Cells [CF]
GSM4509140 Adult Smooth Muscle Cells [SMC]
BioProject PRJNA629778
SRA SRP259919

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE149699_RAW.tar 60.1 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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