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Series GSE149696 Query DataSets for GSE149696
Status Public on May 02, 2020
Title Identification of AflR binding sites in the genome of Aspergillus flavus by ChIP-seq
Organism Aspergillus flavus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report here the AflR binding motif of Aspergillus flavus for the first time with the aid of ChIP-seq analysis. Of the 540 peak sequences associated with AflR binding events, 66.8% were located within 2 kb upstream (promoter region) of translational start sites. The identified 18-bp binding motif was a perfect palindromic sequence, 5′-CSSGGGWTCGAWCCCSSG’3′ with S representing G or C and W representing A or T. On closer examination, we hypothesized that the 18-bp motif sequence identified contained two identical parts (here called motif A and motif B). Motif A was in positions 8–18 on the upper strand, while motif B was in positions 11-1 on the bottom strand. The inferred length and sequence of the putative motif identified in A. flavus were similar to previous findings in A. parasiticus and A. nidulans. Gene ontology analysis indicated that AflR bound to other genes outside the aflatoxin biosynthetic gene cluster.
Overall design Examination of AflR binding sites in A. flavus (three replicates)
Contributor(s) Kong Q, Shan S
Citation(s) 32326370
Submission date May 01, 2020
Last update date Jul 13, 2020
Contact name Qing Kong
Phone +86 13573865136
Organization name Ocean University of China
Department Food Science and Engineering
Street address Yushan Road 5
City Qingdao
State/province Shandong
ZIP/Postal code 266003
Country China
Platforms (1)
GPL23153 Illumina HiSeq 2500 (Aspergillus flavus)
Samples (2)
GSM4509133 AflR_ChIPSeq
GSM4509134 input DNA
BioProject PRJNA629764
SRA SRP264985

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE149696_AflR_vs_input_1_anno_peaks.txt.gz 22.3 Kb (ftp)(http) TXT
GSE149696_RAW.tar 18.2 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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