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| Status |
Public on Sep 18, 2020 |
| Title |
Single Cell RNA-sequencing analysis of the juvenile and embryonic mouse enteric nervous system (ENS) of the small intestine |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The study aimed to make a molecular definition of myenteric neuron classes in the mouse small intestine and describe their differentiation from enteric stem cells during embryogenesis. Method: We performed single cell RNA-sequencing of enteric neurons from small intestine at the juvenile stage (postnatal day (P)21) and ENS cells from the small intestine at embryonic day (E)15.5 and E18.5. We confirmed novel marker genes for all classes using immunohistochemistry and characterised the morphology/axons of three of newly discovered ENS neuron types. Results: 12 unique enteric neuron classes (ENC1-12) were identified and confirmed in native tissue by histochemical methods. The communication machinery (ligand, receptors, adhesion molecules), axonal patterns and morphologies were presented to infer functional roles of the ENCs. Analysis of embryonic transcriptomes revealed that enteric stem cells differentiate into two neuronal branches, that successively diversify into ENC1-7 and ENC8-12, respectively. Conclusion: Utilizing single cell RNA-sequencing we conclude that murine small intestine consists of 12 major myenteric classes that emerge through a step-wise fashion during embryonic development. Reference: Morarach, Mikhailova et al., 2020. BioRxiv 2020.03.02.955757
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| Overall design |
We isolated single myenteric neurons from pan-neuronal Baf53bCre;R26R-Tomato mice (P21) and single ENS cells (including enteric stem cells, glia and neurons) from Wnt1-Cre;R26R-Tomato embryos (E15.5 and E18.5) using flow cytometry and obtained transcriptomes by single cell RNA-sequencing (10xGenomics).
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| Contributor(s) |
Marklund U |
| Citation(s) |
33288908 |
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| Submission date |
Apr 28, 2020 |
| Last update date |
Jun 02, 2021 |
| Contact name |
Ulrika Marklund |
| E-mail(s) |
Ulrika.Marklund@ki.se
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| Phone |
0046702931870
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| Organization name |
Karolinska Institutet
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| Department |
Medical Biochemistry and Biophysics
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| Lab |
Biomedicum C6
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| Street address |
Tomtebodavägen 16
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| City |
Stockholm |
| State/province |
- If in U.S, Select a State - |
| ZIP/Postal code |
SE17717 |
| Country |
Sweden |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA628982 |
| SRA |
SRP258962 |
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