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Series GSE149496 Query DataSets for GSE149496
Status Public on Jul 07, 2020
Title mRNA-seq analysis of oral squamous cell carcinoma (OSCC) cell line OC3 and TW2.6 derived xenografts (CDXs)
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In many solid tumors, tissue of mesenchymal subtype is frequently associated with epithelial-mesenchymal transition (EMT) state, strong stromal infiltration, and poor prognosis. Emerging evidence from tumor ecosystem studies indicate that the stromal components, including cancer associated fibroblast (CAFs), actively participate in cancer growth and progression. Transcriptomic analysis of xenograft tissues provides a unique advantage in dissecting hallmark genes of tumor (human) or stromal (murine) origins.
Overall design For each mouse, a half millions of mycoplasma-free TW2.6 or OC3 cells mixed with equal volume of CorningĀ® MatrigelĀ® matrix (Corning, NY, USA) was subcutaneously implanted into the flank of 10-16 (Exp1) or 11 (Exp2) week-old NOG (National Institute of Infectious Diseases and Vaccinology, Taiwan). We measured the tumor size and mouse weight twice a week. For Exp1, all tumors were collected at day 68. For Exp2, to obtain tumor with ~500 mm3 in size, tumors of TW2.6-NOG were collected at day 32; tumors of OC3-NOG were collected at day 81.
Contributor(s) Su S, Lin C, Lin S
Citation(s) 32605311, 34869001
Submission date Apr 28, 2020
Last update date Oct 18, 2022
Contact name Su-Fang Lin
Organization name National Health Research Institutes, Taiwan
Department NICR
Lab R2-1211
Street address No 35, Keyan Road, Zhunan Town
City Miaoli Conunty
ZIP/Postal code 35053
Country Taiwan
Platforms (2)
GPL19415 Illumina NextSeq 500 (Homo sapiens; Mus musculus)
GPL22245 Illumina HiSeq 2500 (Homo sapiens; Mus musculus)
Samples (11)
GSM4503900 OC3-NOG01
GSM4503901 OC3-NOG03
GSM4503902 OC3-NOG04
BioProject PRJNA628884
SRA SRP258882

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE149496_OSCC_CDX_hg38_cpm.txt.gz 606.2 Kb (ftp)(http) TXT
GSE149496_OSCC_CDX_mm10_cpm.txt.gz 687.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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