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Series GSE149327 Query DataSets for GSE149327
Status Public on Jul 20, 2020
Title RNA-sequencing analysis of tumor-infiltrating B cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Tumour-infiltrating lymphocytes are a major predictor for overall survival and response to immunotherapy for several malignancies. However, while tumour-infiltrating CD8 T cells have been extensively studied, little is known about the role of the humoral immune system within the tumour microenvironment (TME). Here we show that plasma cells are present in the TME and actively secrete human papillomavirus (HPV)-specific IgG antibodies in HPV-positive head and neck squamous cell carcinoma patients. Compared to circulating memory B cells, plasma cells in the TME were enriched for HPV-reactivity with little bystander recruitment of influenza-specific cells. HPV-specific plasma cells in the TME correlated with the antibody titres in peripheral blood and produced antibodies with a high degree of somatic hypermutation directed against HPV E2, E6 and E7 antigens. A striking proportion of HPV-specific antibodies produced in situ were directed against E2, which might thus be a promising target for immunotherapies in HPV-associated cancers. Overall, we show that antigen-specific activated and germinal centre B cells as well as plasma cells can be found in the TME, with plasma cells producing antibodies specific for tumour-associated antigens in situ. These findings provide a better understanding of humoral immune responses in human cancer and suggest that tumour-infiltrating B cells could be harnessed for the development of novel therapeutic agents.
Overall design Antibody secreting cells (ASCs), activated B cells, and germinal center (GC) B cells were sorted from tumors and metastatic lymph nodes of HPV+ HNSCC patients.
Contributor(s) Wieland A, Hudson W
Citation(s) 33208941
Submission date Apr 24, 2020
Last update date Sep 15, 2021
Contact name William Hudson
Organization name Baylor College of Medicine
Department Molecular and Cellular Biology
Street address 1 Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (15)
GSM4497813 PBMC activated B cells (7 days post flu-vaccine) [EVC379_ABC]
GSM4497814 PBMC antibody secreting cells (7 days post flu-vaccine) [EVC379_ASC]
GSM4497815 Metastatic lymph node GC B cells [HPV4LN_ABCpos]
BioProject PRJNA627960
SRA SRP258373

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Supplementary file Size Download File type/resource
GSE149327_RAW.tar 70.8 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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