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Series GSE14921 Query DataSets for GSE14921
Status Public on Apr 07, 2009
Title Hepatocyte-specific deletion of SIRT1 alters fatty acid metabolism
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hepatic metabolic derangements are key components in the development of fatty liver, insulin resistance, and atherosclerosis. SIRT1, a NAD+-dependent protein deacetylase, is an important regulator of energy homeostasis in response to nutrient availability. Here we demonstrate that hepatic SIRT1 regulates fatty acid metabolism by positively regulating PPAR-alpha. Hepatocyte-specific deletion of SIRT1 impairs PPAR-alpha signaling and decreased fatty acid beta-oxidation in the liver. When challenged with a high-fat diet, liver-specific SIRT1 knockout mice develop hepatic steatosis, hepatic inflammation, and endoplasmic reticulum stress. Taken together, our data indicate that SIRT1 plays a vital role in the regulation of hepatic lipid homeostasis.
Overall design ix Lox control and six Liver-specific SIRT1 KO (SIRT1 LKO) mice in 95% C57BL/6 background were fed ad libitum with free access to water and food. They were then sacrificed at 4:00pm to analyze PPAR-alpha mediated phenotypes under physiologically relevant conditions. Livers from these mice were then dissected and RNA was isolated with a Qiagen RNA easy mini kit with on-column DNAseI treatment. RNA quality was validated with the Agilent 2100 Bioanalyzer in the microarray facility. RNA samples were then pooled with 2 animals/replicate and analyzed by Agilent Whole Mouse arrays. Data are generated by the Rosetta Resolver Error Model.
Contributor(s) Purushotham A, Xu Q, Li X, Collins J
Citation(s) 19356714
Submission date Feb 20, 2009
Last update date Jan 12, 2017
Contact name NIEHS Microarray Core
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (6)
GSM371988 Control liver_4 pm_rep1
GSM371989 Control liver_4 pm_rep2
GSM371990 Control liver_4 pm_rep3
BioProject PRJNA111907

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14921_RAW.tar 676.7 Mb (http)(custom) TAR (of TIFF, TXT)
Processed data included within Sample table

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