|
| Status |
Public on Apr 05, 2021 |
| Title |
CPD-seq mapping of UV damage repair in elf1 mutant yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
Elf1 is an important transcription elongation factor that has been implicated in transcription coupled-nucleotide excision repair (TC-NER). Here, we have used a high-throughput sequencing method known as CPD-seq to map the repair of UV-induced cyclobutane pyrimidine dimers (CPDs) at single nucleotide resolution across the yeast genome in elf1 mutant cells. Analysis of CPD repair indicates that Elf1 is important for CPD repair in the transcribed strand (TS) of yeast genes, indicating it plays an important role in TC-NER.
|
| |
|
| Overall design |
UV repair data was analyzed for wild-type (WT) and mutant yeast cells deleted for the ELF1 gene. (i.e., elf1∆). Yeast cells were irradiated with 125J/m2 and allowed to repair for 0 or 2 hours.
|
| |
|
| Contributor(s) |
Wyrick J, Selvam K |
| Citation(s) |
34108662, 39043658 |
| |
| Submission date |
Apr 22, 2020 |
| Last update date |
Aug 16, 2024 |
| Contact name |
John J. Wyrick |
| E-mail(s) |
jwyrick@vetmed.wsu.edu
|
| Phone |
509-335-8785
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
Biotechnology Life Sciences 241
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18249 |
Ion Torrent Proton (Saccharomyces cerevisiae) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA627343 |
| SRA |
SRP257854 |
Less...
More...