Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
Summary
PIWI-interacting RNAs (piRNAs) are genomically-encoded small RNAs that regulate germ cell development and guarantee germline integrity. Mature piRNAs engage Piwi Argonaute proteins to silence complementary transcripts, including transposable elements and endogenous genes. To date, piRNA biogenesis mechanisms are still unclear. Here, we show that the RNA Polymerase II subunit RPB-9 is required to promote transcription elongation at piRNA loci. Through genetic and biochemical experiments, we demonstrate that rpb-9-mediated piRNA production is needed to repress two DNA transposon families and a subset of somatic genes in the C. elegans germline.
Overall design
3 replicates for ChIP-seq, RNA-seq, smallRNA-seq, and 2 replicates for capped RNA-Seq
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