NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE148942 Query DataSets for GSE148942
Status Public on Apr 21, 2020
Title Discrete regulation of β-catenin-mediated transcription governs identity of intestinal epithelial stem cells [scRNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The homeostasis of the gut epithelium relies upon continuous renewal and proliferation of crypt-resident intestinal epithelial stem cells (IESCs). Wnt/β-catenin signaling is required for IESC maintenance, however, it remains unclear how this pathway selectively governs the identity and proliferative decisions of IESCs. Here, we demonstrate that C-terminally-recruited transcriptional co-factors of β-catenin act as all-or-nothing regulators of Wnt-target gene expression. Blocking their interactions with β-catenin rapidly induces loss of IESCs and intestinal homeostasis. Conversely, N-terminally recruited co-factors fine-tune β-catenin’s transcriptional output to ensure proper self-renewal and proliferative behaviour of IESCs. Impairment of N-terminal interactions triggers transient hyperproliferation of IESCs, resulting in exhaustion of the self-renewing stem cell pool. IESC mis-differentiation, accompanied by intrinsic and extrinsic stress signalling results in a process resembling aberrant "villisation" of intestinal crypts. Our data suggest that IESC-specific Wnt/β-catenin output requires discrete regulation of transcription by transcriptional co-factors.
 
Overall design single cell RNA sequencing of small intestinal crypts of betacatenin-mutant mice (3xcontrol, 3xD164A)
 
Contributor(s) Borrelli C, Valenta T
Citation(s) 33649334
Submission date Apr 20, 2020
Last update date Mar 16, 2021
Contact name Tomas Valenta
E-mail(s) tomas.valenta@mls.uzh.ch
Organization name University of Zurich
Department Institute of Molecular Life Sciences
Street address Winterthurerstrasse 190
City Zurich
ZIP/Postal code CH-8057 Zurich
Country Switzerland
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4486372 control0dpi
GSM4486373 control2dpi
GSM4486374 control4dpi
This SubSeries is part of SuperSeries:
GSE148943 Discrete regulation of β-catenin-mediated transcription governs identity of intestinal epithelial stem cells
Relations
BioProject PRJNA626594
SRA SRP257516

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148942_RAW.tar 126.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap