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Series GSE148529 Query DataSets for GSE148529
Status Public on Apr 13, 2020
Title bT87Q-globin gene therapy reduces sickle hemoglobin production, allowing for ex vivo anti-sickling activity in human erythroid cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We performed RNA-seq analysis on immortalized human erythroid cell line (HUDEP-2), that had been engineered to carry the sickle cell disease (SCD) mutation (sHUDEP-2), and on healthy donor mobilized CD34+ derived cells after transduction with a control GFP lentivirus and lentivirus expressing βT87Q-globin. Our results show activation of inflammation- and proliferation-related programs, suggesting minimal changes of background gene expression except for βT87Q-globin expression and endogenous β/βs-globin suppression.
Overall design RNA-seq was performed on non-transduced CD34+ derived human primary cells and on CD34+ derived human primary cells transduced with a control GFP lenitviral vector or βT87Q-globin lentiviral vector at the CD71high/CD235ahigh stage of erythroid development (day 9 of culture) and on non-transduced sHUDEP-2 cells and on sHUDEP-2 cells transduced with a control GFP or βT87Q-globin lentiviral vector at the CD71high/CD235ahigh stage (day 6 of culture).
Contributor(s) Gudmundsdottir B, Tisdale JF, Pirooznia M
Citation(s) 32405513
Submission date Apr 12, 2020
Last update date May 18, 2020
Contact name Mehdi Pirooznia
Phone 410-340-6052
Organization name National Institutes of Health
Street address 12 SOUTH DR
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM4473188 Non-transduced sHUDEP-2 cells
GSM4473189 sHUDEP-2 cells transduced with GFP-lentivirus (MOI50)
GSM4473190 sHUDEP-2 cells transduced with βT87Q-lentivirus (MOI50)
BioProject PRJNA624891
SRA SRP256249

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Supplementary file Size Download File type/resource
GSE148529_Gene_count.txt.gz 450.3 Kb (ftp)(http) TXT
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