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Series GSE14839 Query DataSets for GSE14839
Status Public on Feb 09, 2010
Title Differences in gene expression in islets isolated from pancreata donated after Cardiac Death relative to Brain Death
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The decreasing numbers of Donation after Brain Death (DBD) donors necessitates the comprehensive evaluation of Donation after Cardiac Death Donors (DCD) as a source of pancreata. The aim of this study was to characterize pancreata and islets from DCD and DBD donors with respect to markers of cellular stress that may indicate compromised islet quality. Immunohistochemical staining of pre-isolation pancreas biopsies found increased numbers of caspase 3 positive islets in DBD, while markers of oxidative stress (nitrotyrosine, CML, and HNE) were elevated in DCD. Assessment of islet quality by standard (yield, morphology, fluorescence microscopy, and glucose stimulated insulin secretion) and novel methods (flow cytometry, HPLC quantification of ATP) did not reveal significant differences. However, the post culture loss of DCD islets was increased compared to DBD, and DCD islets showed delayed functional potency when transplanted into diabetic NOD.scid mice. Microarray analysis of cultured islets showed increased expression of multiple stress pathway related genes in DCD compared to DBD. Together these data indicate that the current standard donor management, pancreas recovery and preservation practices are insufficient to quench the oxidative stress injury suffered by DCD islets which leads to loss in culture and may complicate their use in clinical transplant.

Keywords: cell type comparison
 
Overall design In the study presented here, 10 islet preparations isolated from Donation after Brain Death pancreata were compared to 9 sets of islet preparations isolated from Donation after Cardiac Death pancreata. All islet preparations had cold ischemia times less than 11 hours.
 
Contributor(s) Sperger JM, Schug J, White P, Kaestner K, Fernandez LA
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Submission date Feb 13, 2009
Last update date Feb 22, 2018
Contact name Jamie M Sperger
E-mail(s) sperger@surgery.wisc.edu
Organization name University of Wisconsin - Madison
Department Department of Surgery
Lab Lab of Dr. Luis Fernandez
Street address 1111 Highland Ave. RM 5168
City Madison
State/province WI
ZIP/Postal code 53593
Country USA
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (19)
GSM371293 Pancreatic islets_DBD_1
GSM371294 Pancreatic islets_DBD_2
GSM371295 Pancreatic islets_DBD_3
Relations
BioProject PRJNA112107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14839_RAW.tar 178.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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