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Series GSE148294 Query DataSets for GSE148294
Status Public on May 13, 2020
Title HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we demonstrate that HRP2-DPF3a-BAF complex coordinates histone H3 lysine 36 methylation (H3K36me) and ATP-dependent chromatin remodeling to regulate chromatin dynamic and gene transcription during myogenic differentiation. To study the cellular and molecular function of HRP2-DPF3a-BAF complex during myogenic differentiation, we used RNA sequencing (RNA-seq) to generate gene expression profiling during myogenic differentiation under Hrp2/Dpf3a/Brg1 transient knockdown by siRNAs in C2C12. We identified multiple genes whose expression is significantly affected by Hrp2/Dpf3a/Brg1 levels.
Overall design For knockdown condition, C2C12 myoblasts were transfected with siRNAs against Hrp2/Dpf3a/Brg1 or control 24h before start of differentiation. Cells were harvested for gene expression profiling when differentiation was induced (0h, confluent cells) and after 48h of differentiation in differentiation medium. Two replicates were performed per siRNA.
Contributor(s) Zhu X, Lan B, Yi X
Citation(s) 32459350
Submission date Apr 08, 2020
Last update date Jul 20, 2020
Contact name Bingxue Lan
Organization name Tianjin Medical University
Street address Qixiangtai road
City Tianjin
ZIP/Postal code 300070
Country China
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (10)
GSM4459815 MB_siCtrl_rep1
GSM4459816 MB_siCtrl_rep2
GSM4459817 MT_siCtrl_rep1
This SubSeries is part of SuperSeries:
GSE141407 HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription
BioProject PRJNA623796
SRA SRP255718

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Supplementary file Size Download File type/resource
GSE148294_RAW.tar 5.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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