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Series GSE148053 Query DataSets for GSE148053
Status Public on Jun 08, 2020
Title Total RNA sequencing of postnatal male germ cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the whole-transcriptome profile with total RNA sequencing of postnatal male germ cells.
Histone modification levels are dynamically controlled during mammalian spermatogenesis. We found that H3K9 demethylases, Jmjd1a and Jmjd1b catalyze H3K9 demethylation in prospermatogonia. Combined loss of Jmjd1 enzymes disturbed prospermatogonia to spermatogonia transition in mice. To examine a role of Jmjd1 in prospermatogonia to spermatogonia transition, we performed RNA-seq and ChIP-seq analyses using postnatal germ cells at P3 and P7.
 
Overall design mRNA profiles of postnatal male germ cells isolated from testes of a series of Vasa-Cre; Jmjd1; Jmjd1b conditional knockout mice
 
Contributor(s) Kuroki S, Maeda R, Tachibana M
Citation(s) 32679061
Submission date Apr 03, 2020
Last update date Aug 24, 2020
Contact name Ryo Maeda
E-mail(s) rmaeda@fbs.osaka-u.ac.jp
Organization name Osaka University
Street address Yamadaoka 1-3
City Suita
ZIP/Postal code 565-0871
Country Japan
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (14)
GSM4453128 P3_Ctrl_rep1
GSM4453129 P3_Ctrl_rep2
GSM4453130 P3_DKO_rep1
This SubSeries is part of SuperSeries:
GSE148055 RNA-seq and H3K9me2 ChIP-seq of postnatal male germ cells
Relations
BioProject PRJNA622968
SRA SRP255165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148053_RAW.tar 2.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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