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Series GSE14789 Query DataSets for GSE14789
Status Public on Sep 16, 2011
Title An integrative multi-platform analysis for biomarker discovery of osteosarcoma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Three osteosarcoma (OS) cell lines (MG-63, Saos-2 and U-2 OS) and 1 osteoblastic cell line (hFOB1.19) were collected for this work. MG-63 was kindly provided by Dr. Agi Grigoriadis from University College London. Saos-2, U-2 OS and hFOB1.19 were purchased from ATCC. All cells used were kept in exponential phase of growth. Total RNA was extracted using the RNeasy Total RNA Isolation kit (QIAGEN). The quality and purity of the products were controlled by Agilent 2100. The final synthesized biotinylated cDNAs were hybridized to Affymetrix GeneChip® U133A 2.0 arrays following the protocol strictly. Arrays were scanned with the Affymetrix scanner 3000. Data analysis was performed by Microarray Suite 5.0 after pre-standard procedure.
Overall design Link-test on datasets from both SELDI-TOF-MS and microarray high-throughput analysis platforms can accelerate the identification of tumor biomarkers. The results confirmed that CYC-1 with important biomedical function was an effective candidate biomarker for osteosarcoma early diagnosis.
Contributor(s) Li DG, Zhang JW, Zeng zH, Liu LJ
Citation(s) 19445706
Submission date Feb 11, 2009
Last update date Dec 06, 2018
Contact name Long Ji Liu
Phone +8605922187356
Fax +8605922187356
Organization name NEAU
Street address 59# Daxue Road
City Xiamen
ZIP/Postal code 361005
Country China
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM369298 HFOB1 cell line
GSM369299 MG-63 cell line
GSM369300 SaoS-2 cell line
BioProject PRJNA112223

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