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Series GSE147750 Query DataSets for GSE147750
Status Public on Jul 01, 2021
Title OCT4 cooperates with distinct chromatin remodelers in naive and primed pluripotent states in human [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). We mapped the protein-protein interactions of OCT4 in naive and primed hESCs, revealing extensive connections to ATP-dependent nucleosome remodeling complexes. In naive hESCs, OCT4 is associated with both BRG1 and BRM, the two mutually exclusive ATPase subunits of the BAF complex. Genome-wide location analyses and genetic deletion studies reveal that these two enzymes exert a functionally redundant role in transcriptional regulation of blastocyst-specific genes. In contrast, OCT4 cooperates with BRG1 and the transcription factor SOX2 to create an open chromatin architecture at neural lineage-associated genes in primed hESCs. This work offers insight into the regulation of human stem cell identity and reveals how a common transcription factor utilizes differential compositions of the BAF complex in controlling distinct transcriptional programs governing naive vs. primed human pluripotent states.
 
Overall design Genome binding/occupancy profiling of chromatin remodelers BAF155, BRG1, BRM, and transcription factors SOX2 in naive and primed hESCs by ChIP sequencing. Transcriptomes of naive and primed hESCs upon knockdown of either BRG1 or BRM by RNA sequencing.
 
Contributor(s) Huang X, Theunissen TW, Wang J
Citation(s) 34446700
Submission date Mar 30, 2020
Last update date Sep 30, 2021
Contact name Jianlong Wang
Organization name Columbia University Irving Medical Center
Department Department of Medicine
Lab Jianlong Wang
Street address 650 W. 168th St.
City New York
State/province New York
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (11)
GSM4445350 hESC_naive_untransfected
GSM4445351 hESC_naive_ntc
GSM4445352 hESC_naive_brm-1
This SubSeries is part of SuperSeries:
GSE147751 OCT4 cooperates with distinct chromatin remodelers in naive and primed pluripotent states in human
Relations
BioProject PRJNA616309
SRA SRP254636

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Supplementary file Size Download File type/resource
GSE147750_cufflinks.fpkm.tsv.gz 1.3 Mb (ftp)(http) TSV
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Raw data are available in SRA
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