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Series GSE147718 Query DataSets for GSE147718
Status Public on Sep 30, 2020
Title Co-Administration of Propionate or Protocatechuic Acid Does Not Affect DHA-Specific Transcriptional Effects on Lipid Metabolism in Cultured Hepatic Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Long-chain n-3 polyunsaturated fatty acids (n-3 LC-PUFAs) are collectively recognized triglyceride-lowering agents, and their preventive action is likely mediated by changes in gene expression. However, as most studies employ fish oil, which contains a mixture of n-3 LC-PUFAs, the docosahexaenoic acid (DHA)-specific transcriptional effects on lipid metabolism are still unclear. The aim of the present study was to further elucidate the DHA-induced transcriptional effects on lipid metabolism in the liver, and to investigate the effects of co-administration with other bioactive compounds having effects on lipid metabolism. To this purpose, HepG2 cells were treated for 6 or 24 h with DHA, the short-chain fatty acid propionate (PRO), and protocatechuic acid (PCA), the main human metabolite of cyanidin-glucosides. Following supplementation, we mapped the global transcriptional changes. PRO and PCA alone had a very slight effect on the transcriptome; on the contrary, supplementation of DHA highly repressed the steroid and fatty acid biosynthesis pathways, this transcriptional modulation being not affected by co-supplementation. Our results confirm that DHA effect on lipid metabolism are mediated at least in part by modulation of the expression of specific genes. PRO and PCA could contribute to counteracting dyslipidemia through other mechanisms.
Overall design Human liver-derived cell line HepG2 cells were stimulated with vehicle or bioactive compound for 6 hours or 24 hours, respectively, before RNA harvest and subsequent mRNA sequencing (RNA-seq). All samples were performed in biological triplicates.
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Contributor(s) Danesi F, Larsen BD, Di Nunzio M, Nielsen R, de Biase D, Valli V, Mandrup S, Bordoni A
Citation(s) 32993128
Submission date Mar 30, 2020
Last update date Nov 09, 2020
Contact name Alessandra Bordoni
Phone +390512097901
Organization name Università di Bologna
Department Department of Biochemistry and Molecular BiologyDepartment of Agricoltural and Food Sciences
Street address Viale Fanin 44
City Bologna
ZIP/Postal code 40127
Country Italy
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (36)
GSM4443760 SM1810: Control 6hrs, Rep1
GSM4443761 SM1811: Control 6hrs, Rep2
GSM4443762 SM1927: Control 6hrs, Rep3
BioProject PRJNA616255
SRA SRP254537

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Supplementary file Size Download File type/resource
GSE147718_RNA.counts.txt.gz 1.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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